The binding of folate by human serum was investigated by incubating serum with [3H] pteroylglutamic acid followed by charcoal separation of free and bound fractions. Sera from all of 99 normal volunteers and 9 16 patientsexhibitedsome degree of folate binding, ranging from less than 0. 1 to greater than 8 ng/ml. This binding phenomenon was found, by competitive inhibition, to be specific for fo...

Folic acid and folate binding protein (FABP) concentrations were determined in 208 pregnant women as well as in 50 non-pregnant women. Serum folate levels in pregnant women were significantly lower than those of the non-pregnant group. Anaemic pregnant women also had lower serum folate concentrations than those of the non-anaemic pregnant women. Ninetyeight of 208 (47.1%) pregnant women had ser...

The main objective of this study was to assess the association between dietary fiber intake and the folate status of Canadian female adolescents. We also assessed dietary folate intakes and evaluated the prevalence of biochemical folate deficiency in these subjects. Female adolescents aged 14-19 y (n = 224) were recruited and fasting blood samples were collected. Dietary intakes (3-d food recor...

In the present study, the determinants of fasting plasma homocysteine in diabetic subjects were examined; whether plasma homocysteine and vascular disease are related and the influence of the C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene on serum and erythrocyte folate, plasma homocysteine and vascular disease. Diabetic clinic subjects (Type I, n=354; Type II, n=392...

By THOMAS J. LYNCH AND WILLIAM C. MOLONEY D URING STUDIES on the induction of myelogenous leukemia in the rat, serum B12 afl(I folate activities were investigated. In man it is well known tiiat serum B1 activity is greatly elevated in chronic myelogenous leukemia ( CML ) . Less is known concerning serum folate activity hut from iiiitial reports and in our own experience, serum folate levels are...

introduction: there is no comprehensive study on the effect of folate on food intake therefore the present study was carried out to determine the effect of folate supplementation on serum concentrations of leptin and grehlin and food intake in male wistar rats. methods: the study was carried out for 42 days during which the animals were weighed weekly their food and water intakes were measured ...

OBJECTIVE Folate depletion and hyperhomocysteinemia increase the risk for hepatic alcoholic damage and promote oxidative stress in animals. In addition, some investigators have reported an inverse association between serum folate and body mass index and a positive correlation between total homocysteine and fat mass. We investigated whether there is an association between serum folate and total ...

Folate is not stable in serum and plasma. This may impair laboratory diagnostics and distort the outcome of epidemiological studies on folate and chronic diseases. The present study was designed to determine the kinetics of folate loss in human serum and plasma (collected into tubes containing EDTA, heparin, or citrate) at room temperature and the recovery of folate as 4-alpha-hydroxy-5-methylt...

By THOMAS J. LYNCH AND WILLIAM C. MOLONEY D URING STUDIES on the induction of myelogenous leukemia in the rat, serum B12 afl(I folate activities were investigated. In man it is well known tiiat serum B1 activity is greatly elevated in chronic myelogenous leukemia ( CML ) . Less is known concerning serum folate activity hut from iiiitial reports and in our own experience, serum folate levels are...