Epithelial cell infection by Shigella flexneri 2a was studied in an in vitro model system. Using the Henle 407 human intestinal epithelial cell line as host cells, a standardized experimental protocol which allowed quantitative measurement of infection was developed. Intravellular residence of infecting organisms was confirmed by indirect fluorescent-antibody staining of unfixed and methanol-fi...

When a strain of Shigella flexneri and then a strain of Escherichia coli were implanted at an interval of 1 day in the digestive tract of germfree mice, the S. flexneri was eliminated in 8 days. However, when S. flexneri was mono-associated with germfree mice in the absence of E. coli, a population of S. flexneri appeared which was resistant to the antagonistic effects of E. coli in vivo. The e...

ON THE INNER FRONT COVER: Septin‐caged Shigella flexneri in infected HEK293 cells. Bacteria and actin are labelled red, SEPT7 green, DNA blue. Credit: Gizem Ozbaykal‐Guler Serge Mostowy, London School of Hygiene & Tropical Medicine image

SfII is a serotype-converting temperate bacteriophage of the highly prevalent Shigella flexneri serotype 2a. We isolated the SfII phage from a wild-type strain of S. flexneri serotype 2a. Here, we present the complete genome sequence of this phage.

BACKGROUND Shigella is a frequent cause of bacterial dysentery in the developing world. Treatment with effective antibiotics is recommended for shigellosis, but options become limited due to globally emerging resistance. One of the mechanisms for the development of resistance utilizes integrons. This study described the antibiotic susceptibility and the presence of class 1 and 2 integrons in S....

From May 2004 to October 2006, a prospective study was carried out in Dakar, Senegal, to update information about the antimicrobial susceptibility of Shigella spp. isolated from stool specimens. Among the 165 non-duplicate strains collected, 81 (49%) were identified as Shigella flexneri, 75 (45%) as Shigella sonnei, 5 (3%) as Shigella boydii, and 4 (2%) as Shigella dysenteriae. Disk diffusion t...

objective: shigellosis is one of the most common causes of morbidity and mortality in children with diarrhea in developing countries. it is essential to assess the antibiotic resistance patterns of these bacteria. ipah gene is one of the virulence factors which can be used for detection of shigella spp. materials and methods: total of 100 isolates of shigella were collected from different prov...

Introduction: Awareness of the species of Shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. The purpose of this study was to use the Multiplex PCR method and comparison with the culture method to detect Shigella species, as well as analysing the antibiotic resistance pattern in its different species. Methods: Simultaneously, the fe...

INTRODUCTION This study was conducted to determine the prevalence of serotypes, virulence factors, and antimicrobial resistance patterns of Shigella spp. in Beijing, China, from 2004 to 2011. METHODOLOGY Real-time PCR assays were used to detect virulent genes, and the Kirby-Bauer disk diffusion method was used to evaluate antimicrobial resistance. RESULTS Among the total of 1,652 Shigella i...

The serum antibody response to proteins encoded by the virulence-associated plasmid of Shigella flexneri was determined in monkeys challenged with virulent S. flexneri serotype 2a. With water-extractable antigen in an enzyme-linked immunosorbent assay, a significant increase in antibody titer against proteins from a plasmid-carrying, virulent strain of S. flexneri serotype 5 could be demonstrat...