RAPD analysis This study aimed to develop an efficient method for micrpropagation of true-to-type date palm plants through direct organogenesis. Nodular cultures were obtained from shoot tips on MS medium plus 2 mg/l 2ip and 1 mg/l NAA. Among combinations of 2ip and 2,4-D added to culture medium for direct shoot buds proliferation, 5 mg/l 2ip alone gave the highest organogenesis frequency. For ...

This study was aimed to develop a simple, comprehensive and efficiently repetitive protocol for micropropagation of banana (Musa sapientum L.) using shoot meristem. After growing in-vitro cultures on different hormonal combinations, Murashige and Skoog’s medium supplemented with BAP and IAA (5.0 + 1.0 mg/l, respectively) and 10% CW were found to be most efficient and productive combination for ...

A novel protocol for production of multiple shoot tip clumps of Keelar nelli (Phyllanthus amarus Schum. & Thom.) were developed in vitro from shoot tip explants cultured on Murashige and Skoog (MS) medium containing only BAP or BAP with NAA, 3% sucrose and 0.8% agar. The addition of the following combination of growth substances 0.1mgLBAP and 0.05mgLNAA, caused an extensive proliferation of mul...

Vaccinium myrtillus Linn. is an imported fruit tree with high economic value. Currently, in Vietnam there has not been any publication on vitro propagation of the blueberry plant. This study focused V. by nodal culture. The results showed that shoot growth and proliferation were best when nodes cultured WPM medium supplemented 0.5 mg/L zeatin, 30 g/L sucrose, 10 agar, pH 5.8 (shoot height 4.24 ...

The CLAVATA1 (CLV1), CLV2, and CORYNE (CRN) receptors in Arabidopsis thaliana maintain cell proliferation in shoot apical meristems by restricting expression of the transcription factor WUSCHEL (WUS). Previously characterized receptor mutants generate extra fruit and floral organs that are proposed to arise from enlarged floral meristems (FMs). We identified new alleles in clv1, clv2, and crn a...

Halophytes, such as seagrasses, predominantly form habitats in coastal and estuarine areas. These habitats can be seasonally exposed to hypo-salinity events during watershed runoff exposing them to dramatic salinity shifts and osmotic shock. The manifestation of this osmotic shock on seagrass morphology and phenology was tested in three Indo-Pacific seagrass species, Halophila ovalis, Halodule ...

Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7...