Nowadays, many researches were conducted in minimizing tissue culture technology due to the overhead of cost needed. The purpose of this study was to investigate the effects of using five kinds of organic additives at four level concentrations responsive to the number of shoots produced for eight weeks in culture. Stem segment explants of Celosia sp. were cultured on MS medium that have been su...

In vitro regeneration of plants from root cultures of Swertia chirata Buch.-Ham. ex Wall was obtained. Root explants from axenic shoot cultures were used for shoot induction. Optimal shoot regeneration without any callus intervention was observed on 1/2 MS (Murashige and Skoog’s medium, 1962) + 4.44 μM 6, benzylaminopurine (BAP) +1.07 μM α-naphthalene acetic acid (NAA).Regenerated shoots were f...

An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s ...

different explants of crocus sativus l. such as corm, style, ovary, petal, anther and leaf were cultured on ls and ms media supplemented with naa and bap or 2,4-d plus bap at different combinations. shoot and corm induction occurred in addition to the formation of other organs. efficient plantlet regeneration with strong roots occurred at 1 mg 1-i of both naa and bap. an increase in 2,4-d level...

considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...

lisiantus is introduced among the 10 top cutting flowers in the world. micropropagation can be used in this plant because of some problems in traditional propagation. in this research, the effects of the growth regulators on the in vitro shoot regeneration and rooting of the shoots obtained from nodal explants were investigated. the effects of the different concentrations of bap and kin on shoo...

Development of a reproducible, versatile and efficient in vitro plant regeneration system is highly warranted for Indian soybean varieties for their mass multiplication in view of their commercial significance. Accordingly a protocol for direct shoot organogenesis in soybean variety JS 335 has been developed. Using cotyledonary node explants significant organogenic responses, mean shoot number ...

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...