Sections from the human somatosensory cortex were observed with a light microscope. The neurons were classified into light and dark ones. The light neurons were slightly stained with thionin, luxol fast blue MBS and azocarmine G (80% of all neurons). The dark neurons were more or less shrunken, and stained intensely with these dyes (20% of all neurons). Double staining with luxol fast blue MBS ...

Dentin phosphoprotein (phosphophoryn, DPP) is a unique acidic protein in dentin. Except for type I collagen, DPP is the most abundant extracellular matrix protein in dentin. This protein is characterized by high contents of phosphoserine (45-50%) and aspartic acid (35-38%) (Butler et al., 1995)1). Because of the high levels of aspartic acid and phosphoserine, this protein has polyanionic charac...

Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for ...

Silver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR-SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming ...

BACKGROUND The present study is aimed to assess the usefulness of silver nitrate staining of nucleolar organizer regions (NORs) as a quantitative criterion for the diagnosis of selected head and neck tumors. MATERIALS AND METHODS The silver nitrate staining technique was used on 195 paraffin blocks collected from 85 patients. The samples consisted of 21 squamous cell carcinoma (SCC) of larynx...

Blast-induced traumatic brain injury (TBI) is the signature insult in combat casualty care. Survival with neurological damage from otherwise lethal blast exposures has become possible with body armor use. We characterized the neuropathologic alterations produced by a single blast exposure in rats using a helium-driven shock tube to generate a nominal exposure of 35 pounds per square inch (PSI) ...

Nucleolar organising regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23). They are identified by silver staining at low pH. The method also reveals osteocyte canaliculi and cement lines and granules in the cytoplasm of kidney cells in locations that mimic osteopontin distribution. Human bone and kidney sections, benign and lymphomatous p...

The ability of staining slurries containing silver and/or potassium compounds to enhance the mechanical properties of a leucite-reinforced glass ceramic (IPS Empress CAD) was investigated by measuring the Vickers hardness, median crack length, toughness, and compressive residual stress of specimens. A staining slurry containing potassium ions was found to increase the toughness of IPS specimens...