Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeats (ISSRs) markers were performed to detect the genetic diversity among 6 new rice lines and 4 cultivars with different responses to drought tolerance and establish specific DNA markers associated with drought tolerance. Among 16 RAPD primers tested, only 5 produced bands polymorphic between lin...

Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic b...

Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic br...

study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. in the present study, genetic diversity among and within 15 iranian native ajowan(carum copticum l.) populations were determined using inter simple sequence repeat (issr) markers. twelve selected primers produced 153 discernible bands, with 93 (60.78%) being polymorph...

Micropropagated plantlets derived from three different grape rootstock genotypes namely, Dogridge (Vitis champini), SO4 (V. berlandieri×V. rupestris) and ARI-H-144 (V. vinifera×V. labrusca) were subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it large size has a yellow fruit skin color with greenish tips. However, problem production limited amount good quality seeds. The utilization vitro culture techniques on pineapples allows emergence somaclonal variations, especially plantlets that have undergone su...

There is scanty information on tamarind’s genetic diversity in Easter Kenya. The objective of this study was to determine the 64 tamarind accessions from Eastern Kenya using 12 Inter Simple Sequence Repeat (ISSR) markers. DNA extracted young apical leaves modified CTAB method and amplified standard PCR. data collected were scored as presence (1) or absence (0) bands then compared 100bp ladder a...

DNA markers help detect the intraspecific genetic diversity of yeast strains. Eight ISSR (Inter Simple Sequence Repeats) primers were used to assess Saccharomyces cerevisiae (n = 96) from different populations 3), evaluate technological characteristics, and investigate trait-loci associations. The amplified 154 reproducible scorable bands, which 79.87% polymorphic. UPGMA (unweighted pair group ...

Snapmelon (Cucumis melo var. momordica) is native to India and many of its accessions have been used as source for disease and insect pest resistance, worldwide. Inter simple sequence repeat (ISSR) markers were used to evaluate intra-specific genetic diversity among twenty-two snapmelon accessions, variable for fruit cracking, peeling patterns, fruit shape, and flesh colour. Of the 32 ISSR mark...

The utility of inter simple sequence repeat-PCR (ISSR-PCR) assay in the characterization and elucidation of the phylogenetic relationship between the pathogenic and nonpathogenic isolates of Vibrio cholerae is demonstrated. A total of 45 V. cholerae strains including 15 O1 El Tor, nine O139 and 21 non-O1/non-O139 strains were analyzed using eight ISSR primers. These primers, which are essential...