We studied the interaction of the specific DNA aptamer sgc8c immobilized at the AFM tip with its corresponding receptor, the protein tyrosine kinase-7 (PTK7) embedded in the membrane of acute lymphoblastic leukemia (ALL) cells (Jurkat T-cells). Performing single molecule force spectroscopy (SMFS) experiments, we showed that the aptamer sgc8c bound with high probability (38.3 ± 7.48%) and high s...

A method fbr high-perfbrmance liquid chromatography (HPLC) has been estahlished fbr the simultaneous determination of rheinanthrone, sennidin A, scnnidin B and rhein formed by incubation f sennoside A with rat and mouse feces. The analysis was done within 20 min with a Wakosil-II5C18HG column by linear gradient elution using a mobile phase containing aqucous phosphoric acid and acetonitrile. Rh...

The majority of the works dealing with non-isothermal kinetics assumes only one kinetic differential equation, uses linearization techniques and do not check the fit between the simulated and the experimental data. It is not clear from the literature why we need kinetic evaluations at all in this field. Due to this controversial situation, the author has outlined his views on the aims and metho...

The effect of temperature and calcium ions on the denaturation of a recombinant alpha-amylase from Bacillus halmapalus alpha-amylase (BHA) has been studied using calorimetry. It was found that thermal inactivation of BHA is irreversible and that calcium ions have a significant effect on stability. Thus an apparent denaturation temperature ( T (d)) of 83 degrees C in the presence of excess calci...

BACKGROUND Isothermal calorimetry allows monitoring of reaction rates via direct measurement of the rate of heat produced by the reaction. Calorimetry is one of very few techniques that can be used to measure rates without taking a derivative of the primary data. Because heat is a universal indicator of chemical reactions, calorimetry can be used to measure kinetics in opaque solutions, suspens...

Observations of clusters in the 30 to 350 GHz range can in principle be used to determine a galaxy cluster’s Comptonization parameter, y, peculiar velocity, v and gas temperature, Te via the dependence of the kinetic and thermal Sunyaev-Zeldovich (SZ) effects on these parameters. Despite the significant contamination expected from thermal emission by dust in high-redshift galaxies, we find that...