The objective of the present study was to determine the effect of substitution of corn grain with dried citrus pulp (DCP) on milk composition, fatty acids profiles and blood metabolites in Holstein dairy cows. These parameters were measured in a replicated 4 × 4 latin square design experiment using eight Holstein cows. Each experimental period lasted 4 weeks. The four treatments were: control (...

Inbreeding coefficients of artificially bred Ayrshire, Guernsey, Holstein, Jersey, and Brown Swiss cows were calculated from relationships between sires and maternal male ancestors. Percentages of inbred cows and average inbreeding coefficients of inbred cows were: Ayrshire, 26 and 11%; Guernsey, 11 and 4%; Holstein, 31 and 1%; Jersey 23 and 2%; and Brown Swiss, 23 and 2%. Percentages of inbred...

In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF- α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with a...

Progeny of Holstein females mated to sires of different breeds were genetically evaluated along with their purebred Holstein contemporaries born in the same herds using multiple trait animal models. The resulting estimated breeding values (EBV) of cows were averaged within breed of sire and compared relative to progeny of purebred Holstein sires for various economic traits. All progeny were bor...

BACKGROUND Intravenous regional anaesthesia (IVRA) and hindfoot four-point nerve block anaesthesia (NBA) are recommended for local anaesthesia (LA) in the distal limb of dairy cows. Two studies were conducted to compare the efficacy, time until onset and stress responses to IVRA and NBA in dairy cows. In the first cross-over designed study, eight healthy unsedated German Holstein cows, restrain...

The objective of this study was to compare the effects of soybean (SB) meal (SBM) with increased rumen undegradable protein as roasted SB (RSB), extruded SB (ESB) or their equal blend (RSB + ESB) on production performance, nutrient digestibility, N-utilization efficiency, and blood metabolites in mid-lactation dairy cows. Eight lactating Holstein cows (BW = 534 ± 52 and DIM = 104 ± 5; mean ± SD...

A word should be said as to the theoretically important question whether the reciprocal crosses differ in their results. No consistent difference is observable. Eight of the 31 F, cows (indicated by an asterisk in table 1) had Guernsey dams while 23 had Holstein dams. In their first lactation period the cows with Guernsey dams gave less milk but more butter-fat, average 6953 pounds of milk, 287...

Background: Metabolic profiles have been used in efforts to predict pre parturient problems and fertility, to diagnose metabolic diseases, and to assess nutritional status in dairy cows. These profiles may help to confirm the diagnosis of sub clinical diseases, to be aware of possible causes of infertility in the herds, or to monitor improvement in herd animals. OBJECTIVES: This study investiga...

Data from six experiments (two with dry cows) were used to predict partitioning of gross energy to CH4 in Holstein cows using selected independent variables, some of which were intercorrelated, and a stepwise backward elimination regression procedure. Methane outputs ranged from 3.1 to 8.3% (mean 5.5) of gross energy intake for 134 dry cow balance trials and from 1.7 to 14.9% (mean 5.2) of gros...

One of the most pressing challenges is how to take advantage of the unprecedented opportunities that exist today for progress in biomedical research. Recent advances in the understanding of basic cellular and molecular processes provide the opportunity for us not only to unravel the mysteries of normal physiology but also to identify specific defects that contribute to the pathophysiology of va...