The periodate-degradation technique was used to demonstrate the mechanism by which the reducible cross-links of collagen are stabilized. In all the tissues examined, Smith degradations of the 3H-labelled cross-links indicated that dihydroxylysinonorleucine is derived solely from hydroxylysino-5-oxonorleucine, the Amadori-rearranged product of the original condensation reaction. Monohydroxylysin...

The O-polysaccharide (O-antigen) of Escherichia coli O36 was isolated from the lipopolysaccharide and studied by sugar analyses and Smith degradation along with (1)H and (13)C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established, which is unique among the known structures of bacterial polysaccharides: The O-antigen gene cluster of E. coli O36 ...

Gastric-mucosal glycoproteins of sheep have been shown to express the blood-group-I and -i antigens. The highest blood-group-I activities were found in glycoproteins lacking in blood-group-A and -H activities. In antigenically very active glycoprotein preparations, approx. 25% of the macromolecules expressed various blood-group-I and -i antigens, and these could be enriched by affinity chromato...

The chemical structure of the O-antigenic polysaccharide isolated from the lipopolysaccharide of Vibrio cholerae O37 by mild acid hydrolysis was elucidated. The O-antigenic polysaccharide is found to consist of D-glucose, N-acetyl-D-Quinovosamine and small amount of 4-O-methyl-N-acetyl-D-quinovasamine. The structure of the O-antigen is established by using sugar and methylation analyses, Smith ...

A new sialic acid-containing oligosaccharide has been isolated from urine of a patient with a type of mucolipidosis newly recognized by Orii et al. (1972). This compound was found to be composed of galactose (2 moles), mannose (3 moles), N-acetylglucosamine (3 moles) and sialic acid (2 moles). On the basis of the results of sequential glycosidase digestion, of methylation analysis, and of the S...

A novel lipid which contained long-chain base, fatty acid, galactose and N-methylamino-ethylphosphonic acid in an equimolar was isolated from the viscera of Turbo cornutus. The method used for the structural elucidation of this lipid were partial acid hydrolysis, alkaline hydrolysis, periodate oxidation and Smith degradation. The structure of break-down products were mainly identified by combin...

An O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide isolated by the phenol-water extraction from the halotolerant soil bacteria Azospirillum halopraeferens type strain Au4. The polysaccharide was studied by sugar and methylation analyses, selective cleavages by Smith degradation and solvolysis with trifluoroacetic acid, one- and two-dimensional (1)H and (...