A novel approach was developed to quantify rRNA sequences in complex bacterial communities. The main bacterial 16S rRNAs in Drentse A grassland soils (The Netherlands) were amplified by reverse transcription (RT)-PCR with bacterium-specific primers and were separated by temperature gradient gel electrophoresis (TGGE). The primer pair used (primers U968-GC and L1401) was found to amplify with th...

The main purpose of the paper is the numerical analysis of seismic site effects in Caracas (Venezuela). The analysis is performed considering the Boundary Element Method in the frequency domain. A numerical model including a part of the local topography is considered, it involves a deep alluvial deposit on an elastic bedrock. The amplification of seismic motion (SH-waves, weak motion) is analyz...

A comparison was made of the effect of glyphosate (Roundup®Plus), a post-emergency applied herbicide, and of Harness®GTZ, a pre-emergency applied herbicide, on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize. The potential effect was monitored by direct amplification, cloning and sequencing of soil DNA encoding 16S rRNA, rhizobacterial DNA hybridization to...

Site amplification factors at period ranges of 0.1-0.2, 0.2-0.3, 0.3-0.5, 0.5-1, 1-2, and 2-10 s were determined for 27 stations of KiKnet and K-NET in and around the source area of the 2008 Iwate-Miyagi Nairiku earthquake. The ratio of the geometric mean values of the two maximum horizontal amplitudes of acceleration and velocity at the uphole to that at the downhole is considered as the ampli...

In order to investigate the genetic diversity of streptomycetes in an acid forested soil sample from Mt. Coot-tha, Brisbane, Australia, cells were mechanically lysed within the soil matrix and genomic DNA was isolated and purified. 16S ribosomal (r)DNA was amplified by the polymerase chain reaction (PCR) method using one primer conserved for members of the domain Bacteria and a second designed ...

The effect of buffer composition on simultaneous PCR amplification of 16S rRNA gene fragments of five bacterial species was examined using a number of different buffer systems. Tris-based PCR buffers at final concentrations of 10 mM proved unreliable. However, when the final concentration of Tris was increased to 75 mM, all five samples were routinely detected. The use of other buffers, 3-[(1,1...