Electrochemical cleaning of oil-contaminated soils is a promising area environmental safety, as it can be easily organized even in locations remote from settlements. For this purpose, power source and system electrodes are necessary equipment. It possible to use an electric generator if there no supply lines nearby. The material affects the features redox processes, which affect energy consumpt...

Hydroxylation reactions play important roles in many biosynthetic and catabolic processes. The mechanism of enzymatic hydroxylation reactions, however, has not been fully understood, mainly because the enzymes that catalyze such reactions are particulate or unstable and the extensive purification has met with great difficulties. In a preliminary report (2), we described the isolation from a soi...

Purification and PCR amplification procedures for DNA extracted from environmental samples (soil, compost, and river sediment) were improved by introducing three modifications: precipitation of DNA with 5% polyethylene glycol 8000 (PEG) and 0.6 M NaCl; filtration with a Sepharose 4B–polyvinylpolypyrrolidone (PVPP) spin column; and addition of skim milk (0.3% w/v) to the PCR reaction solution. H...

The present study was aimed at purification and characterization of α-amylase enzyme from strain of Aspergillus niger isolated from local soil samples. Solid state fermentation (SSF) was carried out to produce α-amylase from A. niger using waste banana peel as substrate. The maximum activity of α-amylase (11926 U/gds) was recorded after 72 hours of fermentation. The extracted enzyme was subject...

Molecular analyses for the study of soil microbial communities often depend on the extraction of DNA directly from soils. These extractions are by no means trivial, being complicated by humic substances that are inhibitory to PCR and restriction enzymes or being too highly colored for blot hybridization protocols. Many different published protocols exist, but none have been found to be suitable...

A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide seque...

Feathers are byproduct waste of poultry processing plant and produced in large amount. A small percentage of feather waste is steamed, chemically treated, ground, to form feather meal a dietary protein supplement for animals. Alternatively, keratin can be biodegraded by some Keratinolytic bacteria and in this study Keratinase producing bacteria and their Keratinolytic enzyme production was inve...

Enzyme-linked immunosorbent assay (ELISA) and sol-gel-based immunoaffinity purification (IAP) methods for the pyrethroid bioallethrin were developed and applied for monitoring bioallethrin in spiked food, soil, and dust samples. Attempts to determine bioallethrin content in fruit and vegetable extracts revealed high variability between sample preparations and marked interferences with the assay...

Due to the annual increasing volumes of oil and petroleum products extraction refining, probability ecosystem pollution with heavy hydrocarbons is increasing. At present, it biological methods soil purification that are most promising from both an ecological economic point view. The main mining regions characterized by a hot climate high salinity soil. It known effectiveness use destructive mic...