A validated, multigene-based method using real-time quantitative PCR (qPCR) and the Razor Ex BioDetection system was developed for detection of Phymatotrichopsis omnivora. This soilborne fungus causes Phymatotrichopsis root rot of cotton, alfalfa, and other dicot crops in the southwestern United States and northern Mexico, leading to significant crop losses and limiting the range of crops that ...

Lopez-Nicora, H. D., Simon, A. C. M., Dossman, B. C., Paul, P. A., Dorrance, A. E., Lindsey, L. E., and Niblack, T. L. 2015. Distribution and abundance of Heterodera glycines and Macrophomina phaseolina in Ohio. Plant Health Prog. 17:35-41. Soybean and corn are grown on more than 60% of the arable land in Ohio. The soybean cyst nematode (SCN) is responsible for significant losses incurred by so...

trichoderma harzianum (t39), t.virens (dar74290), t.viride (mo), t. harzianum (m) and trichderminb a commercial formulation of t. harzianum(bi) were evaluated as potential biological agents for the control of charcoal stem rot in melon caused by macrophomina phaseolina. cell-free metabolites of t. harzianum(m), t. harzianum (t39) and t. virens (dar 74290) inhibited growth of m. phaseolina compl...

Evaluation of Rhizoctonia zeae as a potential biological control option for fungal root diseases of sugar beet" (2015). Abstract Several common root diseases routinely damage sugar beet in Nebraska and other production areas of the Central High Plains, and it is becoming more common to find fields infested simultaneously with multiple pathogens. Owing to the shortage of available fungicides for...

Over the last 10 years plant pathologists have begun to realize that more knowledge about the genetic structure of populations of plant pathogens is needed to implement effective control strategies (48). Research on the genetic structure of fungal populations has mushroomed, and review papers that summarize these studies are numerous (7,27,33,34,38). Although the number of fungal studies has in...

ABSTRACT The spatial distribution and temporal development of tomato crown and root rot, caused by Fusarium oxysporum f. sp. radicis-lycopersici, were studied in naturally infested fields in 1996 and 1997. Disease progression fit a logistic model better than a monomolecular one. Geostatistical analyses and semivariogram calculations revealed that the disease spreads from infected plants to a di...

Pseudomonas aureofaciens 30-84 is a soilborne bacterium that colonizes the wheat rhizosphere. This strain produces three phenazine antibiotics which suppress take-all disease of wheat by inhibition of the causative agent Gaeumannomyces graminis var. tritici. Phenazines also enhance survival of 30-84 within the wheat rhizosphere in competition with other organisms. Expression of the phenazine bi...

Rhodococcus equi is a facultative intracellular, Gram-positive, soilborne actinomycete which can cause severe pyogranulomatous pneumonia with abscessation in young horses (foals) and in immunocompromised people, such as persons with AIDS. All strains of R. equi isolated from foals and approximately a third isolated from humans contain a large, ~81-kb plasmid which is essential for the intramacr...

ABSTRACT Diagnosis of yellow vine disease (YVD) in cucurbits, an important disease in the south-central United States, relies on external symptom appearance, phloem discoloration, and the presence of bacterium-like organisms (BLOs) in phloem. Polymerase chain reaction (PCR) amplification of BLO nucleotide sequences was explored as a means to improve diagnostic techniques. PCR, using a primer pa...

In biological control studies of plant-parasitic nematodes in field soil improved methods are needed for reducing or eliminating specific soil inhabiting microorganisms. Microwave heating of soil decreases soilborne fungi and bacteria, but not Pasteuria spp. Bacterial and fungal colony forming units were reduced to nondetectable levels in microwaved heated field soft (650 watts) at 5.2% moistur...