Soluble guanylate cyclase (sGC) is the mammalian endogenous nitric oxide (NO) receptor. The mechanisms of activation and deactivation of this heterodimeric enzyme are unknown. For deciphering them, functional domains can be overexpressed. We have probed the dynamics of the diatomic ligands NO and CO within the isolated heme domain β(1)(190) of human sGC by piconanosecond absorption spectroscopy...

Background Protective effects of NO, a physiological activator of soluble guanylyl cyclase (sGC), have been reported in ischemia/reperfusion syndrome (I/R) of the lung. However, its protective effects can be overwhelmed by its deleterious effects due to oxidative products formation. Therefore, we studied the effects of direct sGC stimulator (BAY 41-2272) and sGC activator (BAY 58-2667) on I/R i...

Nitric oxide signals through activation of soluble guanylyl cyclase (sGC), a heme-containing heterodimer. NO binds to the heme domain located in the N-terminal part of the β subunit of sGC resulting in increased production of cGMP in the catalytic domain located at the C-terminal part of sGC. Little is known about the mechanism by which the NO signaling is propagated from the receptor domain (h...

Beet pulp as component of cattle feed can be processed in different way. Tis study was performed to characterize the physical properties of so called fine beet pulp (FBP); normal beet pulp (NBP) and pelleted beet pulp (PBP). The following parameters were determined: bulk density, kinetics of hydration, functional specific gravity (FSG), water holding capacity (WHC), soluble dry matter and intri...

This paper reviews the use of texture analysis in studying the performance of hydrophilic matrices of highly soluble drugs and different types of excipients (i.e. water-soluble, water-insoluble and swellable, and water insoluble and non-swellable). Tablets were prepared by direct compression, and their swelling and erosion in presence of these different excipients were assessed with the help of...

Two multi-phasic models (logistic (LOG) and Michaelis-Menten (MM)) with three sub-curves were used to describe gas production kinetics of corn (CG), barley (BG), wheat (WG) and triticale (TG) grains. In each model sub curve, 1 describes the gas production caused by fermentation of the soluble fraction, gas production caused by fermentation of the non-soluble fraction is described in sub curve 2...

The impact of three levels of sea salt (0.0, 8.0, and 16.0 mS/cm2) on six varieties of soybean (Crawford, G21, G22, G35, G82, and G83) was studied. Growth criteria, photosynthetic pigments, soluble sugars, soluble protein, free amino acids, free proline, and protein profile of soybean varieties were investigated under sea salt stress. Results of this study showed a considerable decrease in grow...

The effect of soluble fluorine species in different concentrations on the catabolic activity of Desulfotomaculum ruminis bacteria has been studied. The fluoride concentrations tolerated by and toxic to the bacteria have been determined. The degree of fluorine species toxicity was found to depend on the form in which fluorine is introduced into the medium and increases in the following order: Na...