Coupled folding and binding of intrinsically disordered proteins (IDPs) is prevalent in biology. As the first step toward understanding the mechanism of binding, it is important to know if a reaction is 'diffusion-limited' as, if this speed limit is reached, the association must proceed through an induced fit mechanism. Here, we use a model system where the 'BH3 region' of PUMA, an IDP, forms a...

1. Introduction The last decade has seen a considerable increase in the number of biochemical and biomedical applications of PFG NMR diffusion methods. However, despite commercial availability of powerful, stable, and reproducible gradient units, 1 H diffusion measurements of low-concentration solutes in non-deuterated media can still present a challenging problem. Large solvent peaks can make ...

The amorphous polymer film swelling in a liquid solvent below the glass transition temperature was characterized by a few kinetic parameters (especially the mutual diffusion coefficient of swelling and its mean value) obtained by interference of monochromatic light in the wedge arrangement. This interferometric method allows one to determine the concentration field in the swollen surface layer ...

Diffusion and sorption of Biodegradable blends of sodium alginate (SA) and lignosulphonic acid (LS) has been studied at room temperature by conventional weight gain experiments. The sorption studies of SA/LS blends are carried in pH medium of 7.4. The sorption data are used to evaluate the mass transfer coefficients such as intrinsic diffusion coefficient and permeation coefficient. It was obse...

An alternative derivation of Brownian motion is presented. Instead of supplementing the linearized Navier-Stokes equations with a fluctuating force, we directly assume a Gaussian action functional for solvent velocity fluctuations. Solvating a particle amounts to expelling the solvent and prescribing a boundary condition to the solvent on the interface that is shared with the solute. We study t...

Molecular dynamics simulations are performed of bovine pancreatic trypsin inhibitor in a cryosolution over a range of temperatures from 80 to 300 K and the origins identified of elastic dynamic neutron scattering from the solution. The elastic scattering and mean-square displacement calculated from the molecular dynamics trajectories are in reasonable agreement with experiments on a larger prot...

Experimental determinations of the dynamics of photoinduced electron transfer from rubrene to duroquinone in three solvents, dibutyl phthalate, diethyl sebacate, and cyclohexanone are presented. Measurements of the donor (rubrene) fluorescence decays were made with time-correlated single-photon counting. The data are analyzed using recent theoretical developments that include important features...