We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical e...

We incorporate newly developed solid-state detector technology into time-resolved laser Raman spectroscopy, demonstrating the ability to distinguish spectra from Raman and fluorescence processes. As a proof of concept, we show fluorescence rejection on highly fluorescent mineral samples willemite and spodumene using a 128×128 single-photon avalanche diode (SPAD) array with a measured photon det...

BACKGROUND After resection of long-segment Hirschsprung's disease, severe perianal dermatitis (SPAD) may occur because of high stool frequency and elevated concentrations of fecal pancreatic proteases. We investigated prospectively the effect of potato-derived protease inhibitors (PPI) on skin conditions in children with postoperative SPAD. METHODS Four children (aged 12 to 24 months) with th...

A highly accurate method for the determination of the detection efficiency of a silicon single-photon avalanche diode (Si-SPAD) is presented. This method is based on the comparison of the detected count rate of the Si-SPAD compared to the photon rate determined from a calibrated silicon diode using a modified attenuator technique, in which the total attenuation is measured in two attenuation st...

sCMOS imagers are currently utilized (replacing EMCCD imagers) to increase the acquisition speed in super resolution localization microscopy. Single-photon avalanche diode (SPAD) imagers feature frame rates per bit depth comparable to or higher than sCMOS imagers, while generating microsecond 1-bit-frames without readout noise, thus paving the way to in-depth time-resolved image analysis. High ...

Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime. Here, a 32 × 1 single-photon avalanche diode (SPAD) array was explored as a detector for fluorescence lifetime imaging (FLIM) in HCS. Device parameters and characterization results were interpreted in th...

To extract the sensitive bands for estimating the winter wheat growth status and yields, field experiments were conducted. The crop variables including aboveground biomass (AGB), soil and plant analyzer development (SPAD) value, yield, and canopy spectra were determined. Statistical methods of correlation analysis, partial least squares (PLS), and stepwise multiple linear regression (SMLR) were...

The appearance of miniaturized single-photon detectors has enabled massively parallel conversion of photons to digital signals that can be readily processed and/or transported with negligible losses. As a consequence, a new class of imaging sensors is emerging, known collectively as all-digital image sensors, for detection and processing of ultra-fast and complex photonic events, often present ...

Nitrogen (N) in plants is generally diagnosed by a soil test and plant tissue analysis.However, such analyses are costly in terms of time and money and are not easily accessible byresearchers and extension workers, let alone farmers. Alternative cost-effective methods arerequired for rapid analysis of the N status of crops and to guide N management in wheat. Theobjective of this study was to as...