Phophodiesterase (PDE) isoenzyme profiles of human cell preparations and tissues have been analysed by a semiquantitative method using selective PDE inhibitors and activators. Neutrophils, eosinophils and monocytes contain PDE IV exclusively. Lymphocytes, alveolar macrophages and endothelial cells contain PDE IV and PDE III, and in addition, PDE I is measured in macrophages and PDE II in endoth...

Malate dehydrogenase, α-esterase and β-esterase isoenzymes have been analyzed by native polyacrylamide gel electrophoresis in different tissues (liver, kidney, muscle and hump) of the Arabian Camel, Camelus dromedaries in order to study the tissue specificity of these isoenzymes. Malate dehydrogenase recorded three fractions while both esterases recorded two fractions in different studied tissu...

Serum lacticodehydrogenase (LDH) is commonly increased in patients with haematopoietic malignancies and has been shown to be a prognostic factor in patients with non-Hodgkin's lymphoma (NHL) and myeloma. We have examined the LDH isoenzyme content in serum of 326 patients, including 252 patients with NHL (202 at diagnosis and 50 at relapse), 28 patients with Hodgkin's disease, 17 patients with C...

x > c) .3 0.6 ...c) Vanaman, T. C., Sharief, F. & Watterson, D. M. (1977) in Walsh, M. P., Vallet, B., Autric, F. & Demaille, J. G. (1979) J . Biol. Calcium-Binding Proteins and Calcium Function (Wasserman, R. H., Corradino, R. A., Carafoli, E., Kretsinger, R. H., MacLennan, D. H. & Siegal, F. L., eds.), pp. 107-116, North-Holland, Wong, P. Y. K. & Cheung, W. Y. (1979) Biochem. Biophys. Res. Am...

Fig. 1. Electropherogram stained for alkaline phosphatase isoenzymes A. albumin with added biiWubin; B, billrubin solution In saline; C, patient’s serum with artifactual band in addition to placental and lIver isoenzyme bands (poody resolved); D. intestinal and placental isoenzyme control; E, patient’s serum with liver Isoenzyme band; F, Intestinal and live’ lsoenzyme control; 0, patient’s seru...

The morphological and cytochemical changes in erythroid-cell differentiation have been extensively studied: application of the velocity-sedimentation technique has provided preliminary data on the associated biochemical changes (Denton & Arnstein, 1973). After the last cell division there is a dramatic increase in the synthesis of haemoglobin which, at least in part, probably accounts for the a...

THE recent interest in organ-specific patterns of lactate dehydrogenase (LDH) isoenzymes (WIELAND and PFLEIDERER, 1957; WROBLEWSKI, Ross and GREGORY, 1960; PLAGEMANN, GREGORY and WROBLEWSKI, 1960; WROBLEWSKI and GREGORY, 1961) stimulated search for isoenzyme pattern differences among regions of the brain. VAN DER HELM (1962) found no isoenzymic differences among several regions of the human bra...

The isoenzyme pattern of LDH does not play a physiological role; except perhaps during fast transitions in energy metabolism. Aging, 3(5), 457-460. Introduction: A paper describing increased brain lactate concentration with age recently caught our attension [1]. The proposed explanation for the increased brain lactate with age was a shift in the brain lactate dehydrogenase (LDH) isoenzyme patte...

Serum and tumor tissue of a patient with neuroblastoma contained an abnormal isoenzyme of lactate dehydrogenase (LDH; EC 1.1.1.27), which, on agarose gel electrophoresis, migrated between LDH-2 and LDH-3 with a mobility the same as that of the extra LDH isoenzyme found in normal human erythrocytes. On surgical removal of the tumor, the high total LDH activity (775 U/L) in the serum of the patie...

We describe a simple, rapid immunoaffinity procedure for purifying the MB isoenzyme of creatine kinase. Immunoaffinity gel is prepared by linking a monoclonal antibody ("Conan-MB"), specific for this isoenzyme, to Sepharose 4B. Heart tissue is homogenized and fractionated with 40-70% saturated ammonium sulfate before it is applied to the immunoaffinity gel. CK-MB activity, retained on the gel, ...