The ability to cryopreserve spermatozoa from all of the domestic species is challenging. Even though all of the cells must endure similar physical stresses associated with the cryopreservation processes, sperm from the different species are very different in size, shape and lipid composition, all of which affect cryosurvival. Thus, when a cryopreservation protocol has been optimized for sperm o...

Background: Since the deleterious effects of reactive oxygen species (ROS) during sperm cryopreservation can be overcome by antioxidants, post-thaw human sperm quality may perhaps profit from adding antioxidants to the extender media prior to cryopreservation. This study evaluated the effects of different concentrations of Trolox supplementation to cryo-protective media on post-thaw motility an...

In the abomasal epithelium of the goat, glycoconjugates have been studied by means of selective histochemical methods. The staining procedures employed were alcian blue (AB) pH 1.0 AB pH 2.5, Periodic acid Schiff (PAS) and AB pH 2.5-PAS. In addition twelve lectins, Triticum vulgaris WGA, Concana valin A, Glycine max SBA, Dolichos biflorus DBA, Arachis hypogea PNA, Solanum tuberosum STL, Datura ...

Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender, active substances within the egg yolk which protect sperm against cryoinjury remain to be discovered. Among its compositions, low-density lipo...

the objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin c and e as antioxidants. pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/ml with citrate –egg yolk (cey) or tris – egg yolk (tey) extenders. the diluted semen was divided to 5 aliquots including control and 4 experimental groups....

Mammalian spermatozoa are coated with a thick glycocalyx that is assembled during sperm development, maturation, and upon contact with seminal fluid. The sperm glycocalyx is critical for sperm survival in the female reproductive tract and is modified during capacitation. The complex interplay among the various glycoconjugates generates numerous signaling motifs that may regulate sperm function ...

Background. Cryopreservation of spermatozoa with the best morphofunctional characteristics may allow their use for assisted reproductive technologies in goat breeding throughout year to effectively increase livestock. Aim. To evaluate influence seasons on morphological and morphometric fresh cryopreserved sperm. Methods. The ejaculate samples three sexually mature bucks Saanen breed were obtain...

objective(s):the aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development. materials and methods: tissue sections from days 15 to 20 of wistar rat embryos and 1 to 12 postnatal days of rat newborns including developing eye were prepared for lectinhistochemistry technique. horseradish peroxidase (hrp)-label...

Success in cryopreserving stallion semen has been very variable. Several different freezing regimes have been published. However, because extenders and procedures used in each regime have differed, direct comparison of these techniques has been very difficult, and controlled studies comparing different techniques have not been reported. A number of different factors affect sperm cryosurvival. I...

BACKGROUND A transient state of azoospermia may occur due to toxic, environmental, infectious or iatrogenic conditions. Finding sperm in the ejaculate of such patients is often unpredictable and may be critical in IVF treatment. In the present study, the approach of pooling and cryopreservation of sperm is evaluated. Cryopreservation was performed in a unique group of patients in whom no sperm ...