Bovine sperm protease, 66 kDa (BSp66) is a serine protease previously characterized in bovine spermatozoa. Like other proteases, it may be present in sperm from other mammalian species different from bovine, playing a role in the fertilization process. In this study, we looked for BSp66 in hamster spermatozoa using heterologous antibodies against bovine BSp66. An immunoreactive protein was dete...

Sperm morphological analysis (SMA) is an essential step in diagnosing male infertility. Using images of human sperm cells, this research proposes a unique sequential deep-learning method to detect abnormalities semen samples. The proposed technique identifies and examines several components sperm. In order conduct study, we used the online Modified Human Morphology Analysis (MHSMA) dataset cont...

The post-coital test evaluates the penetration of spermatozoa into cervical mucus; it relies on subjective measurements and therefore lacks precision. Enzymatic liquefaction of cervical mucus allows sperm concentration to be measured in post-coital test samples, but the reliability of such measurements is not known. Donor cervical mucus was used as a model to test the accuracy and sensitivity o...

The aim of this study was to investigate whether a relationship exists between the presence of low numbers of leukocytes in normal ovulatory cervical mucus and sperm quality and lipid content after migration. The percentages of live, motile and morphologically normal spermatozoa, movement parameters assessed by computer-aided sperm analysis (CASA), and ionophore-induced acrosome reaction measur...

Bull fertility and semen quality are of paramount importance to the cattle industry. Fluorescent biomarkers measured by flow cytometry can aid in the detection of sub-fertile bulls as they can identify abnormal spermatozoa that may not be properly identified as inferior with standard methods of subjective semen analysis. This paper introduces the basics of flow cytometry, discusses fluorescent ...

Infertility is one of the diseases in which researchers are interested. disease a global health concern, and andrologists constantly looking for more advanced solutions this disease. The intracytoplasmic sperm injection (ICSI) process considered as most common procedures achieving fertilization. Sperm selection performed using visual assessment dependent upon skills laboratory technicians such ...

OBJECTIVE To evaluate the effect of cryopreservation on sperm motility and viability and to assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation before and after cryopreservation in liquid nitrogen. DESIGN Analysis of cryopreservation effects on sperm DNA integrity. SETTING Laboratory of Histology-Embryology of medicine faculty, Sfax, Tunisia. PATIENT(S...

The frequency of recombination between the G gamma-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing greater than 700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allele-specific oligonucleotide hybridization. Our maximum likelihood recombination fraction estimat...

Human papilloma virus (HPV) is one of the most common viral sexually-transmitted diseases worldwide. The prevalence of HPV is higher in infertile males when compared with fertile men and ranges between 10 and 35.7% in men affected by unexplained infertility. HPV can bind to spermatozoa and can potentially be transferred to fertilized oocytes. Viral detection in blastocysts and trophoblastic cel...

The aim of this study was to investigate the roles of oxygen free radicals and mitochondrial signaling in semen disorders, in particular, how this induces low concentrations and reduced motility of sperm. Ejaculate samples were obtained from 120 young adult males (mean age, 28.7±5.3 years) with normal semen (n=30), oligospermia (n=30), asthenospermia (n=30) and oligoasthenozoospermia (n=30). Th...