Internal standardization was applied to the solid-phase extraction of trace elements using following commercially available aminocarboxylic acid-type chelating resins: InertSep ME-2, NOBIAS Chelate PA-1, and Presep PolyChelate. The concentration in initial sample solution can be calculated by ratio added amount internal standard element, Y, that final after extraction, which is proportional vol...

A precise quantification of insect chitin is needed in order to avoid overestimation crude protein due chitin-bound nitrogen. An UPLC/FLR method was optimized and validated for the determination glucosamine (GlcN) hydrolyzed from materials. The applied quantifying content mealworms (Tenebrio molitor) crickets (Acheta domesticus). baseline separation obtained using an Acquity HSS T3 C18 column, ...

Realizing personalized medicine, which promises to enable early disease detection, efficient diagnostic staging, and therapeutic efficacy monitoring, hinges on biomarker quantification in patient samples. Yet, the lack of a sensitive technology and assay methodology to rapidly validate biomarker candidates continues to be a bottleneck for clinical translation. In our first direct and quantitati...

Phytohormone analysis using liquid chromatography combined with tandem mass spectrometry is a very important tool for studies involving plant metabolism; however, this analysis can be affected by matrix composition. Although it is necessary to understand this effect to produce reliable results, it has been widely neglected in analyses of plant hormones. Leaf extracts from Bauhinia variegata wer...

the present study describes a simple, accurate, precise and cost effective uv-vis spectroscopic and rp-hplc method for the estimation of ibandronate sodium (ibn). the determination of ibandronate sodium (ibn) was performed by both uv and rp-hplc method using 215 nm as the determination wavelength. the drug was dissolved in naoh solution (0.1n naoh) for estimation in uv and in distilled water fo...

Isotopic analyses of a total-spiked sample by multiple-collector inductively coupled plasma mass spectrometry (MCICP-MS) are often corrected for mass fractionation by doping the sample with an external tracer element of known isotopic composition and similar mass. However, because of the potential for elemental fractionation on the ICP-MS, internal normalization methods should be used whenever ...

These lines are the intersections of the plane through the point p in the world and the centres of the left and right cameras with the two camera focal planes (see Figure 1). These epipolar lines are formed by the intersections of the pencil of planes through the line joining the image centres with the camera focal planes. Hence such lines form a pencil passing through the point of intersection...

BACKGROUND Despite the increasing use of real-time PCR in the diagnosis and management of viral infections, there are no published studies adequately addressing the optimum number of calibrators, the number of replicates of each calibrator, and the frequency with which calibration needs to be repeated. This study was designed to address these issues. METHODS Cycle threshold data (ABI 7700) wa...

This work describes a procedure for the determination of copper, selenium, and zinc in serum using the Agilent 7500ce collision/reaction cell (CRC) ICP-MS in both helium and hydrogen modes. The method utilizes the high-accuracy technique of isotope dilution whereby the sample is spiked with an isotopically enriched solution to an optimum ratio and measured alongside a spiked calibration standar...

Reverse transcription (RT) followed by a polymerase chain reaction (PCR) represents the most powerful technology to amplify and detect trace amounts of mRNA (Heid et al., 1996; Lockey, 1998). To quantify these low abundant expressed genes in any biological matrix the real-time quantitative RT-PCR (qRT-PCR) is the method of choice. Real-time qRT-PCR has advantages compared with conventionally pe...