The relationship between the way in which normal hemopoietic stem cells respond to irradiation alone or in the presence of bleomycin sulfate (BLM-S) and actinomycin 0 (ACT-D) was investigated. Single doses of BLM-S at 0.3 mg/kg and ACT-O at 0.10 mg/kg body weight were injected intravenously 1-6 hours prior to whole body irradiation and treatment was repeated twice more with time intervals....

Highly purified murine granulocyte-macrophage progenitor cells (CFU-GM) were used as target cells to assess the possible direct effects of purified preparations of recombinant murine gamma-interferon, prostaglandin E, recombinant human heavy chain (acidic) ferritin, and recombinant human tumor necrosis factor alpha (TNF-alpha) on progenitor cells in vitro. Target CFU-GM, with cloning efficienci...

are characteristic of the early disease induced by this virus (3, 17, 18, 30). Late in the course of Friend virus infection, cells have been demonstrated in the spleen which have the capacity for continuous proliferation when transplanted into normal hosts or cultivated in vitro (8, 16, 19, 20, 34). But whether the infected cells acquire these neoplastic character istics late in the disease or ...

Mouse marrow cells were exposed to 5-fluorouracil (FU) either in vivo or in vitro and the effects on the hematopoietic stem cell compartment were studied. The drug was highly toxic to bone marrow cells including the spleen colony-forming unit (CFU-S) population. The small population of stem cells surviving FU, however, caused a different pattern of spleen colony growth when injected into lethal...

The effect of melphalan on mouse myeloma and hemopoietic progenitor cells was studied with a primary cell culture assay for mouse myeloma stem cells developed by Park, Bergsagel, and McCulloch and bone marrow cell culture and spleen colony assays for hemopoietic progenitor cells. Myeloma and bone marrow cells were exposed in vivo to graded doses of melphalan and harvested 3 and 24 hr later for ...

Introduction: The current study investigated the combination of high Z atoms (iodine-, platinium-based drugs) with using low energy irradiation (120kvp) in Anaplastic Thyroid cancer cells.Materials and Methods: For this purpose, eight groups were designed: control (CNT), different concentrations of Iodine contrast media (ICM),  irradiation with various doses, Cis-platin (CDDP) with different c...