background and aims: in spite of a long tradition of fruit-tree growing in all provinces of iran, information on pear (pyrus communis) tree viruses is scant. in order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. materials and methods: leaf samples of pear showing virus like symptoms were col...

among the viruses infecting honey bees, chronic bee paralysis virus (cbpv) is known to induce significant losses in honey bee colonies. cbpv is an unclassified polymorphic single stranded rna virus. using rt-pcr, the virus infections in honey bees can be detected in a rapid and accurate manner. bee samples were collected from 23 provinces of iran, between july-september 2011 and 2012. a total o...

objective: schizophrenia is a complex widespread neuropsychiatric disorder. this illness encompasses a complex debilitating mental disorder causing illusion, delusion, disturbed relationship, low motivation and decline of emotion. viral infection of the brain including borna disease virus (bdv) may play a role in transient or permanent neurological and behavioral abnormalities. this role of bor...

background classification of cancer subtypes by means of microarray signatures is becoming increasingly difficult to ignore as a potential to transform pathological diagnosis nonetheless, measurement of indicator genes in routine practice appears to be arduous. in a preceding published study, we utilized real-time pcr measurement of indicator genes in acute lymphoid leukaemia (all) and acute my...

objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...

Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more sensitive and faster molecular methods hav...

The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...