Background: Theileria annulata is predominant and of utmost economical importance tick borne pathogen bovines in the region which routinely diagnosed based on microscopic examination Romanowsky stained thin blood smears. Present study was intended to evaluate analyze detection efficacy a commercial polymerase chain reaction kit assay comparison conventional PCR classical microscopy for T. from ...

Background and Objectives: Sputum smear staining for acid-fast bacilli is initial approach to the diagnosis of pulmonary tuberculosis (PTB) but more than 50% of cases are sputum smear-negative. This study was aimed to investigate the diagnostic value of fiberoptic bronchoscopy (FOB) guided bronchoalveolar lavage (BAL) in patients suspected to have tuberculosis. Methods: This prospective cross-...

BACKGROUND & OBJECTIVES Sputum acid-fast bacilli (AFB) microscopy services are not available in all health facilities. Alternative procedures are needed to transport sputum samples to the diagnostic centres for detection of AFB. The objective of the present study was to evaluate sputum smears made by pot-method with the direct smears made immediately after sputum collection by Ziehl-Neelsen (ZN...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

BACKGROUND To quantify the likely impact of recent WHO policy recommendations regarding smear microscopy and the introduction of appropriate low-cost fluorescence microscopy on a) case detection and b) laboratory workload. METHODOLOGY/PRINCIPAL FINDINGS An audit of the laboratory register in an urban hospital, Lilongwe, Malawi, and the application of a simple modelling framework. The adoption...

Fluorescein isothiocyanate-labelled goat antihuman immunoglobulin (anti-IgG, anti-IgA, anti-IgM) and anticomplement conjugates were used to detect antibody or complement-coated bacteria from the oropharynx of patients with pharyngitis. Throat smears prepared from patients with a positive culture for group A beta-hemolytic streptococci had significantly more bacteria that stained with labelled a...

BACKGROUND Intraoperative smear cytology provides a rapid and reliable intraoperative diagnosis and guidance to the neurosurgeon during surgical resection and lesion targeting. It also helps the surgeon to monitor and modify the approach at surgery. OBJECTIVES 1) To assess the utility of intraoperative smear cytology and correlate with the final histopathological diagnosis. 2) To describe the...

A polymerase chain reaction (PCR) protocol for detecting IS6110 repetitive insertion sequence of Mycobacterium tuberculosis (MTB) was tested on archival Papanicolaou (Pap)-stained fine needle aspirated (FNA) smears from 24 patients with cervical tuberculous lymphadenopathy and 30 negative controls. The protocol involved protease digestion or phenolchloroform extraction, and simple or nested PCR...

Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next analyzed by trichrome staining of both unconcentrated and concentrated specimens...

Aim: In this study, the phenomenon of oral exfoliative cytology is explored and buccal squamous cells are examined for intracytoplasmic, Prussian blue stained, iron containing granules. addition, these subjected to morphometric measurements their nuclear cytoplasmic diameter along with ratios. Methods: It was a Descriptive, Cross sectional study including 120patients Thalassemia Major, ranging ...