l c t a The accidental importation of plant pathogens in or on the bodies of parasitoids imported as natural enemies has been raised as a potential risk of classical biological control projects involving insects that serve as vectors of plant diseases. During quarantine evaluation of two parasitoids, Tamarixia radiata Waterston and Diaphorencyrtus aligarhensis (Shafee, Alam and Agarwal), import...

Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymerase chain reaction (RT-PCR). This study aimed identify robustness a colorimetric RT-LAMP assay kit that we developed, detecting SARS-COV-2 viral RNA within 30 minutes using primer set special N gene against RT-PCR, gold...

Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard. A recent study showed that supercoiled circular confirmation of DNA appeared to suppress PCR amplification. However, to what extent to which different structural types of DNA (cir...

introduction butyrivibrio fibrisolvens strains are presently recognized as the major butyrate-producing bacteria found in the rumen and digestive track of many animals and also in the human gut. in this study we reported the development of two dna based techniques, quantitative competitive (qc) pcr and absolute based real-time pcr, for enumerating butyrivibrio fibrisolvens strains. despite the ...