The superantigen staphylococcal enterotoxin A (SEA) binds to major histocompatibility complex (MHC) class II molecules at two sites on either side of the peptide groove. Two separate but cooperative interactions to the human class II molecule HLA-DR1 were detected. The first high affinity interaction to the DR1 beta chain is mediated by a zinc atom coordinated by H187, H225, and D227 in SEA and...

The regulation of staphylococcal enterotoxin A (SEA) synthesis in a defined medium was studied using continuous culture techniques. SEA production was repressed by glucose and repression could be overcome by addition of exogenous cyclic AMP. As well as this classical catabolite repression control, addition of glucose to de-repressed steady-state cultures resulted in rapid disappearance of toxin...

OBJECTIVE The aim of this study was to construct a conditionally replicating adenovirus pPE3-SEA expressing staphylococcal enterotoxin A (SEA) gene. MATERIALS AND METHODS A full-length SEA gene fragment was cloned into pENTR12 plasmid to obtain a recombinant viral plasmid pENTR12-SEA. The pENTR12-SEA plasmid was co-transfected into HEK293 cells along with pPE3-ccdB, which encoded for the viru...

Staphylococcus aureus is the most prevalent bovine mastitis pathogen in Argentina. The ability of this organism to produce enterotoxins is linked to staphylococcal food poisoning. Staphylococcal enterotoxins are low molecular weight proteins, highly resistant to heat and proteolytic enzyme activity. The aim of this study was to determine the ability to produce enterotoxins and types of enteroto...

The in vitro exposure of staphylococcal enterotoxin B to trypsin resulted in the formation within 30 min of a product (enterotoxin-T) unchanged in molecular weight, but with threonine as a second NH2 terminus. Upon reduction of the -SSbridge in enterotoxin-T, two fragments were separated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The peptide bond between Lys-97 and Thr-98 ...

the present study was aimed to identify the frequency of coagulase (coa) and thermonuclease (nuc) genes and staphylococcal enterotoxin a (sea) production among staphylococcus aureus isolated from various sources in shiraz. moreover, the correlation between the sea gene and coagulase and thermonuclease enzymes is also considered. a total of 100 s. aureus were isolated from various sources includ...

In this study, the enterotoxigenic potential of Staphylococcus strains (n = 574) isolated from raw milk samples (n = 140) was determined for their capacity to produce staphylococcal enterotoxins. In addition, the relationship between the presence of enterotoxins, coagulase, and thermonuclease (Tnase) was assessed. The results showed that 19% of Staphylococcus was enterotoxigenic, being able to ...

Sugiyama, H. (University of Chicago, Chicago, Ill.), and E. M. McKissic, Jr. Leukocytic response in monkeys challenged with staphylococcal enterotoxin. J. Bacteriol. 92:349-352. 1966-The feeding of staphylococcal enterotoxin to monkeys elicited a leukocytosis which was evident within 0.5 hr of challenge. The peak neutrophilic leukocytosis was reached in 3 hr, and then subsided so that leukocyte...

BACKGROUND In the previous studies using the commercial ELISA kit, the existence of staphylococcal superantigens has been reported in synovial fluid of patients with rheumatoid arthritis (RA). OBJECTIVES This study aimed to design molecular methods to detect staphylococcal enterotoxin C in synovial fluid of patients with rheumatoid arthritis. MATERIALS AND METHODS In this experimental study...

Although several species of animals have been reported to be susceptible to staphylococcal enterotoxin, Dack (1956) stated ". . . no experimental animal has been found that will react to the small amount of enterotoxin sufficient to cause severe reactions in man." Of the few animals regularly used to determine the presence of toxin in test samples of material, the Macaca mulatta monkey fed by s...