A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method wa...

Kolář M., J. Bardoň, I. vágnerová, P. Sauer, d. Koukalová, J. Petrželová, l. Čekanová, r. Pospíšil: resistance to antibiotics in Strains of Staphylococcus spp., Enterococcus spp. and Escherichia coli Isolated from rectal Swabs of Pigs. acta Vet Brno 2008, 77: 103-1100. The study aimed at determining the level of resistance of selected bacterial species (Staphylococcus spp., Enterococcus spp., E...

This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-3-phosphate deh...

This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-3-phosphate deh...

Bacterial sepsis is one of the major causes of morbidity and mortality among neonates. It is characterized as generalized bacterial infection indicated by positive blood culture in the first four weeks of life (Agarwal et al., 2001). The common causative agents for neonatal sepsis are Staphylococcus spp, Enterococci spp, E. coli, Klebsiella spp, Enterobacter spp, Pseudomonas aeruginosa and Acin...

Mastitis and antimicrobial resistance are a big challenge to the dairy industry in sub-Saharan Africa. A study was conducted in Kashongi and Keshunga subcounties of Kiruhura District (in Uganda) where the government and private sector have deliberate programs to improve production efficiency, quality, and safety of milk and its products. The study aimed to determine the prevalence of mastitis, ...

Bacterial pathogens are becoming more and more resistant to antibiotics used commonly by healthcare practitioners. Despite introduction of new antibiotics, empiric treatment of patients with bloodstream infections (BSIs) became a major challenge for practicing physicians.1,2 To depict the susceptibility patterns of BSI pathogens endemic in a referral pediatric hospital, Children’s Medical Cente...

This study was conducted to generate microorganisms isolated from cultures of ears taken from the patients diagnosed to have chronic otitis media and determine and compare the in vitro effects of different antibiotics on these microorganisms. Between June 2009 and October 2010, 127 ear cultures were taken from 100 patients who applied to the Department of Otolaryngology of Ataturk University Fa...

Present study was undertaken to determine the relative incidence of different micro-organisms in postoperative wound infections and their in vitro antibiotic sensitivity. Swab samples from 50 patients were examined. The per cent incidence of different micro-organisms was: Gram-negative bacilli 33.34, Staphylococcus spp. 30.67, Bacillus spp. 13.33, Streptococcus spp. 12.00, Candida spp. 5.33 and...

CSOM is mostly caused by bacteria, but fungi and virus can also be a cause of CSOM. The aerobic microorganisms most frequently isolated in CSOM are Pseudomonas aeruginosa, Staphylococcus aureus, Gram-negative organisms such as Proteus spp., Klebsiella spp., and Escherichia spp., Citrobacter species, Haemophilus influenzae, and Moraxella catarrhalis (Gül et al., 2006; Verhoeff et al., 2006; de M...