Current protocols for the culture of human zygotes to blastocysts use two-step sequential media systems. The efficacy of a one-step system involving potassium simplex optimized medium (KSOM(AA)) has been investigated. In study 1, development of zygotes from days 1 to 3 in KSOM(AA) was compared with that for medium P-1. In study 2, embryos were cultured from days 1 to 3 in P-1 followed by cultur...

production of new hybrids is important for growing aquaculture and protecting wild stocks of fish species. a hybrid between female asp × male kutum ( leuciscus aspius ♀ × rutilus frisii ♂) was produced and its growth performance was monitored in two rearing systems for 55 days. the fish held in earthen ponds exhibited higher performance compared to those held in concrete tanks. the results also...

STUDY QUESTION Can complete oocyte development be achieved from human ovarian tissue containing primordial/unilaminar follicles and grown in vitro in a multi-step culture to meiotic maturation demonstrated by the formation of polar bodies and a Metaphase II spindle? SUMMARY ANSWER Development of human oocytes from primordial/unilaminar stages to resumption of meiosis (Metaphase II) and emissi...

Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental potential and quality of bovine SCNT embryos cultured in different two-step culture media based on KS...

of building cultural centers in all communities and with any kind of attitude has always been one of the designers’ issues. when we use the term in ordinary daily conversation, we often think of culture as an equivalent to the “higher things of the mind”- art, literature, music and painting. according to sociologists, the concept of culture includes such activities, but also far more. culture c...