Severe congenital neutropenia is one of primary immunodeficiency disorders that characterized by severe neutropenia and is associated with severe systemic bacterial infections from early infancy. Granulocyte colony stimulating factor (GCSF) is clinically used as a treatment for congenital and acquired neutropenia. The aim of this study was evaluation of GCSF (PD- Grastim) in treatment of these ...

The objective of this study was to determine if the inverse relationship between perceived physical fitness (pFIT) and exercise frequency (ExFreq) levels and chronic inflammation and oxidative stress exists after making statistical adjustments for confounders including body mass index (BMI), age, gender, and cigarette smoking. Study participants (60% female and 40% male; n = 998) varied widely ...

Alzheimer's disease (AD) and frontotemporal dementia (FTD) are two major causes of dementia. These diseases both progressive neurodegenerative whereas no curative treatment has not yet been established. Regenerative approaches have extensively researched for AD FTD, but they still in early phase pre-clinical trials. We show here, the first time, that cytokines such as hepatocyte growth factor (...

the present study aimed at investigating the beneficial effects of co-administering granulocyte colony–stimulating factor (g-csf) and stem cell factor (scf) in a model of chronic liver injury induced by thioacetamide (taa). biochemical and histopathology- cal examinations were performed on serum and liver specimens. at the end of the treatment period, the rats were anesthetized with ether, seru...

Retinopathy of prematurity (ROP) is a disorder of neonatal retinal vascularization. The incidence is increasing in developing countries like India in view of the rising numbers of preterm deliveries and improved neonatal care. Traditional modalities of treatment included cryotherapy and laser therapy, which were laborious and required special training. Hence, research is on way to find novel tr...

Granulocyte colony-stimulating factor (G-CSF)-producing nonhematopoietic malignancies have been reported in various organs and are associated with a poor clinical outcome. Moreover, carcinoma of unknown primary site (CUP) is an uncommon malignancy that occurs in about 2-6% of cancer patients. CUP also has a poor prognosis due to its missing profile. Since both G-CSF-producing carcinoma and CUP ...

PEGylation of therapeutic proteins has signifi cantly improved their value by altering the physicochemical and biological properties such as increased solubility, decreased immunogenicity, increased half-life, and protection against proteases. Size Exclusion Chromatography (SEC) is the method of choice for determining impurities with molecular mass higher than that of PEGylated proteins. SEC of...

A fibroblast-mediated gene delivery method was used for the endogenous expression of human granulocyte colony-stimulating factor (G-CSF) as a model for cytokine supplement therapy. Human G-CSF cDNA was inserted into the plasmid expression vector BMGNeo, which contains a partial sequence of bovine papilloma virus and a selectable marker gene. The recombinant plasmid (BMGNeo-GCSF) was transfected...