Many streptomycetes strains are hardly or not at all transformable via protoplasts, there is a problem with the regeneration of protoplasts. We found that protoplasts formed directly in cultivation media under submerged conditions presence lytic enzyme. Actinophage ?1/6 endolysin and lysozyme were used this study. Streptomyces cultivated several glycine enzyme for 24 48h. The highest amounts (a...

Our earlier studies on transcriptional signals of mycobacteria had revealed that (i) strong promoters occur less frequently in the slowly growing pathogen Mycobacterium tuberculosis H37Rv than in the fast-growing saprophyte M. smegmatis and (ii) mycobacterial promoters function poorly in Escherichia coli. We now present evidence that RNA polymerases of M. smegmatis, M. tuberculosis, and M. bovi...

Gomis-Ruth F X , Maskos K , Betz M , Bergner A , Huber R , Suzuki K , Yoshida N , Nagase H , Brew K , Bourenkov G P , Bartunik H and Bode W (1997) Mechanism of inhibition of the human matrix metalloproteinase stromelysm 1 by TIMP 1, Nature 389 pp 77 81 Willenbrock, F and G Murphy (1994) Structure-Function Relationships in the Tissue Inhibitors of Metalloprotemases American Journal of Respirator...