The random amplified polymorphic DNA (RAPD) technique has been used widely for purposes such as the construction of linkage maps, QTL analysis, evaluation of genetic diversity, and parentage tests. However, some minor bands of RAPD display a low reproducibility and low reliability. We describe a simple and efficient method for making minor RAPD bands clearer. The first step of this method is to...

Background. Anthracnose black rot caused by the phytopathogenic fungus Colletotrichum acutatum Simmonds became an extremely harmful disease of strawberries in Southern Russia. The is widespread throughout world, and relatively recently it appeared Yield losses due to reach up 80%; besides, pathogen causes significant plant mother plantations. most reliable protection against cultivation resista...

Presently there is no vaccine against Neisseria gonorrhoeae and therefore accurate information on gonococcal transmission plays a crucial role for interventions designed to limit the spread of infections caused by this microorganism. We evaluated the impact of two different categories of genetic markers, (i) concatenated sequences of 10 housekeeping genes and (ii) hypervariable porB DNA sequenc...

The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selectio...

Abstract Aim Operative numbers are critical in applications to Core and Higher Surgical training, ARCP CCT requirements. However, instructions for the use of supervision codes on surgical eLogbook ISCP ambiguous. This study seeks determine if there is a consensus coding common general operations. Method A survey 10 operative vignettes was distributed doctors who used eLogbook. Participants were...

Aberrant crypt foci (ACFs) are the earliest identifiable epithelial lesions thought to precede the development of a subset of colon tumors. To assess their predictive value to adenoma development, we have tested in mice whether the development of ACFs and adenomas is controlled by the same genes. Therefore we used the CcS/Dem series of recombinant congenic strains, in which the effect of multip...

This study examined how supine-to-stand (STS) performance is related to process and product assessment of motor competence (MC) in children. Ninety-one children aged 5–9 years were assessed for process and product MC (10 m running speed and standing long jump) as well as process and product measures of STS. Tertiles were created for STS process and STS product scores to create 3 groups reflecti...

Steroid sulfatase (STS) is localized in the endoplasmic reticulum and catalyzes desulfation of 3beta-hydroxysteroid sulfates. X-linked ichthyosis (XLI) is an inherited skin disorder caused by deficiency of STS enzyme activity. We previously reported a case in which XLI with a one-base change in the STS gene and variation in amino acid Q560P developed. In this study, we performed molecular analy...

For the first time, we used multilocus sequence typing (MLST) to understand how Romanian group B streptococcus (GBS) strains fit into the global GBS population structure. Colonising isolates recovered from adult human females were tested for antibiotic resistance, were molecularly serotyped based on the capsular polysaccharide synthesis (cps) gene cluster and further characterised using a set o...