hemoglobin is a tetrameric oxygen transport protein in animal bodies. however, there is a paucity of information regarding differences between alpha and beta subunits of hemoglobin in terms of oxygen affinity. the sequential model of koshland, nemthy and filmer (knf model) has attributed similar affinities to both alpha and beta subunits. the main purpose of the present study is to construct a ...

background:the zinc-finger x linked (zfx) gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. due to the role of zfx in cell growth, understanding zfx protein-protein interactions helps to clarify its proper biological functions in signaling pathways. the aim of this study is to define zfx protein-protein interactions and the role of zfx in cell growth. m...

introduction: there is considerable evidence that glutamate-mediated excitatory neurotransmission plays an important role in mediating the behavioral actions of acutely administered ethanol. the aim of the present study was to investigate the effect of acute ethanol exposure on nr1 subunit of nmda (n-methyl-d-aspartate) receptor distribution in the cerebral cortex of chick embryo on the 10th an...

The transferrin receptor of Trypanosoma brucei (TbTfR) is a heterodimer glycosylphosphatidylinositol (GPI)-anchored ESAG6 subunit and an ESAG7 subunit. To investigate whether the GPI-anchor essential for function TbTfR, with transmembrane domain instead (ESAG6tmd) was inducibly expressed in bloodstream form trypanosomes. It shown that ESAG6tmd able to dimerise TbTfR can bind transferrin. Fracti...

The studied area, located in the south-western part of Gorce Mountains, is built flysch deposits representing Krynica and Bystrica Subunits Magura Nappe. Here, Late Cretaceous–Early Miocene lithostratigraphic succession Subunit, four formations containing several members were distinguished, whereas only one Middle Eocene–Oligocene formation was identified. Turbacz Kudłoń Thrust Sheets tectonic ...

background and purpose: trichosporon is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. the aim of this work was to study the isolation, identification and molecular analysis of trichosporon species in soil. materials and methods: in order to isolate and identify trichosporon species in soil, 30 samples were collect...

background: herpes simplex virus type 2 (hsv-2) is highly prevalent and major cause of genital herpes in humans. the life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting hsv disease. hsv glycoprotein d (gd) is one of the most important viral immunogen which has an essential ...

background: cholera toxin b subunit (ctb) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. the aim of this study was to compare the expression of native and mutant recombinant ctb (rctb) in pqe vector. methods: ctxb fragment from vibrio cholerae o1 atcc14035 containing the substitution of mutant ctxb for ami...

objectives: enterotoxigenic escherichia coli (etec) is one of the most common agents of diarrhea among other bacterial agents. designing and producing vaccine against these bacteria is one of the major purposes of world health organization (who). due to presence of diverse clones of etec strains in the world, the use of global vaccines for etec infection is controversial. b subunit of heat labi...