For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide...

Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans. With a view to reducing the number of experiments and obtaining the mutual interactions between the variables, a 24 full-factorial central composite design was employed for experimental design and analysis of the results. Four independent ...

Sulphuric acid adsorbed on silica gel is a practical and useful catalyst for acetylation of varius alcohols, Phenols and amines.

In the present study, dormancy and germination requirements were investigated in seeds of Citrullus colocynthis (L.). Seeds were subjected to 10 pretreatments in the present study.  These pretreatments included hot water (100° C) followed by the seeds placed between double layered filter paper moistened with 5 ml of distilled water and kept at 28 - 30 °C, sulphuric acid (98% v/v), for 30 min fo...

There is a widespread interest in the commercial application of D-glucose-isomerase for the large-scale conversion of D-glucose into fructose-containing syrups[ ]. As such, there is a need for a rapid and reliable method for the routine analysis of glucose-isomerising activity in fermentation broths. The analytical methods currently in use include the incubation of the enzyme preparation with a...