We demonstrate a novel, low-cost method of building a surface plasmon resonance and fluorescence (SPRF) microscope with high quality SPR and evanescent-field fluorescence imaging using a laser pointer and an oscillating scatterer. SPR Microscopy and SPR fluorescence provide two detection modalities in a highly compact inexpensive package. Applications include point-of-care diagnostics with sens...

We demonstrated an enhanced surface plasmon resonance (SPR) detection by incorporating a nanoporous gold film on a thin gold substrate. Nanoscale control of thickness and roughness of the nanoporous layer was successfully accomplished by oblique angle deposition. In biosensing experiments, the results obtained by biotin-streptavidin interaction showed that SPR samples with a nanoporous gold lay...

Surface plasmon resonance (SPR) is an established method for studying molecular interactions in real time. It allows obtaining qualitative and quantitative data on interactions of proteins with lipid membranes. In most of the approaches, a lipid membrane or a membrane-mimetic surface is prepared on the surface of Biacore (GE Healthcare) sensor chips HPA or L1, and the studied protein is then in...

We present an numerical investigation of a planar waveguide surface plasmon resonance (SPR) sensor in silicon-oninsulator (SOI) for detecting a change in the imaginary part of the refractive index. By adding a thin adsorbed layer between the metal-analyte boundary it is found that an enhanced sensitivity of the sensor is obtained, suggesting the possibility of realizing a highly integrated and ...

The resolution of conventional surface-plasmon-resonance (SPR) imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave SPR fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF provides lateral resolution approaching 100 nm and offers the advantages of significant sign...

Introduction In this study, we describe the results of controlled synthesis and application of gold coated Fe3O4/SiO2 nano-shells combined with the optical property of gold for enhancement of selective photothermal interaction with cancerous cells based on the surface plasmon resonance (SPR) Phenomena. Materials and Methods Magnetite Nano-Particles (MNPs) were prepared by means of co-precipitat...

We investigated grating-coupled transmission-type surface plasmon resonance (SPR) for sensing applications. In the transmission-type SPR structure, propagating surface plasmons are outcoupled to radiation modes by dielectric and metallic gratings on a metal film. The results calculated in air and water suggest that the proposed structures present extremely linear sensing characteristics. In ter...

A novel surface plasmon resonance (SPR) biosensor which is capable of monitoring proteomic biomarker secretion from living cells is reported here. Vascular endothelial growth factor (VEGF) secretion from living SKOV-3 ovarian cancer cells was measured for concept demonstration.

A novel method for real-time and inline wetness measurement based on surface plasmon resonance (SPR) is presented in this paper. The Kretschmann geometry is adopted to excite surface plasmon waves in our measurement system. In order to prevent water coating, an ultrathin layer of hydrophobic coating is formed on the surface of Au layer. The experimental results show that the level of steam wetn...

Dual-mode surface-plasmon resonance (SPR) sensors use both long- and short- range surface plasmon waves to differentiate surface binding interactions from interfering bulk effects. We have optimized the design of these sensors for minimum surface limit of detection (LOD) using a Cramer-Rao lower bound for spectral shift estimation. Despite trade-offs between resonance width, minimum reflectivit...