Methods commonly used for covalent immobilization of single stranded DNA have been applied to several solid supports (Sephadex G-25 and Cellex 410) as well as to a number of macroporous materials (Sepharose C1-6B, C1-2B; Sephacryl S-500 and S-1000). Coupling efficiencies and stability of covalently bound DNA are compared for both classes of materials. The yields of the immobilization reaction f...

The extent of evolutionary conservation of DNA complimentary to RNA stored in the mature oocyte of the sea urchin S. purpuratus has been assessed. To do this, such DNA was hybridized with total genomic DNA of S. purpuratus and S. franciscanus and the thermal stability of the resultant duplexes was measured by two methods. In the first method, the duplexes were bound to hydroxylapatite and therm...

The hOCTN1 (human organic cation transporter 1) overexpressed in Escherichia coli and purified by Ni-chelating chromatography has been reconstituted in liposomes by detergent removal with a batch-wise procedure. The reconstitution was optimized with respect to the protein concentration, the detergent/phospholipid ratio and the time of incubation with Amberlite XAD-4 resin. Time-dependent [(14)C...

embryonic stem cells are capable of differentiating to variety of cell tissues including cardiomyocytes. this developmental change is accompanied with a great deal of ion channel expression and functions. mouse stem cell derived cardiomyocytes were prepared and separated to yield isolated single cell suspension for cell current recording. in the present study some properties of the k + -current...

T HE tetraethylammonium ion has been used quite extensively since 1946, when Acheson and Moe1' 2 demonstrated its action as a blocking agent, effective at both sympathetic and parasympathetic ganglions. Earlier, Burn and Dalei and Hunt,4 had described the actions of certain quaternary ammonium bases which included the tetraethylammonium ion.* This drug has been used therapeutically with varying...

Recordings were obtained from neurons in layer II/III of slices of rat frontal cortex maintained in vitro. We investigated whether brief application of the potassium channel blocker tetraethylammonium (TEA), which induces a novel form of synaptic plasticity in the CA1 region of the hippocampus referred to as LTPK, evokes similar responses in neocortex. Consistent with previous findings, TEA pro...

This work aims to identify a suitable electrolyte composition for the operation of zinc–nickel flow cell at ambient temperature. The effect varying containing KOH, ZnO, tetraethylammonium hydroxide (TEAH) or tetrabutylammonium bromide (TEAB) additives are investigated. A 15 mM concentration TEAH is found provide smooth and compact zinc depositions. Increasing concentrations KOH be detrimental v...