The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...

background: frozen- thawed embryo transfer is an essential part of art treatment and outcomes of this procedure are associated with several clinical factors. several studies have showed an increase level of ivf outcomes in women with sufficient vitamin d. objective: whether treatment of vitamin d insufficiency can improve pregnancy rates in frozen-thawed embryo transfer cycles. materials and me...

Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chip. Our study is aimed at exploring a novel device for ultra-rapid cryopreservation of small numbers of spermatozoa without cryoprotectant based on polydimethylsiloxane (PDMS) chips. Sper...

Background: In vitro fertilization (IVF) has become increasingly common, today representing about 3% of all live births in some countries. Most patients have to undergo more than one treatment in order to achieve a live birth. Thus cumulative live birth rates are highly interesting to the patients. The most important health problem in IVF is the high rate of multiple births, leading to increase...

ObjectiveTo determine whether the ooplasm granulation patterns of donor oocytes, like those oocytes from poor-prognosis patients, are predictive in vitro fertilization (IVF) outcomes.DesignRetrospective cohort study.SettingAcademically affiliated private clinical infertility and research center.Patient(s)770 fresh 381 vitrified-thawed metaphase II young donors (aged 21.0–34.6 years) used for IV...

current ruminant feeding systems depend on knowledge of the composition of feeds and the rate and extent of degradation of feed organic matter (om) and crude protein (cp) in the rumen. the effect of storage and preparation of samples on in vitro gas production and fermentation characteristics of two common forage species, namely alfalfa and ryegrass werestudied. samples were prepared as fresh (...

INTRODUCTION A 3D-nanofiber scaffold acts in a similar way to the extracellular matrix (ECM)/basement membrane that enhances the proliferation and self-renewal of stem cells. The goal of the present study was to investigate the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on frozen-thawed neonate mouse spermatogonial stem cells (SSCs) and testis tissues. METHODS The isolated sper...

Cumulative pregnancy rate has been significantly increased since frozen-thawed embryo transfer was applied in ART cycles. This method has become an essential part of IVF/ICSI treatment. Luteal phase support has been proven to be associated with higher rate of live birth rate. Human chorionic gonadotropin (HCG), and progestrone have been successfully used for luteal phase support in ovarian stim...

High-latitude soils store vast amounts of perennially frozen and therefore inert organic matter. With rising global temperatures and consequent permafrost degradation, a part of this carbon stock will become available for microbial decay and eventual release to the atmosphere. We have developed a simplified, two-dimensional multi-pool model to estimate the strength and timing of future carbon d...

The effects of meat shape and size, freezing method and thawing temperature on the drip loss of beef brisket and the protein content of its thaw exudates were examined in this study. The beef brisket was cut into the following shapes: cube; brick-shape with rectangle cross-section; and brick-shape with square cross-section and were then frozen using a standard freezer (-15 oC), an air-blast fre...