Francisella tularensis is one of the most deadly bacterial agents, yet most of the genetic determinants of pathogenesis are still unknown. We have developed an efficient targeted mutagenesis strategy in the model organism F. tularensis subsp. novicida by utilizing universal priming of optimized antibiotic resistance cassettes and splicing by overlap extension (SOE). This process enables fast an...

background carbapenemes are a good choice for treatment of infections caused by multidrug resistant pseudomonads aeruginosa. the emergence of carbapenem resistance has become a major problem in treatment of this organism especially among immunocompromised patients including burn patients. objectives the aim of this study was to investigate carbapenem-resistance mechanisms among burn patients in...

We improve Levenshtein’s upper bound for the cardinality of a code of length four that is capable of correcting single deletions over an alphabet of even size. We also illustrate that the new upper bound is sharp. Furthermore we construct an optimal perfect code that is capable of correcting single deletions for the same parameters.

There are lots of condom brands, sizes, and pleasure-inducing gimmicks out there ? but it's not always easy to find which one will work best for you. When beginning to explore sexual intimacy, roadblocks like yours may arise, but the fact that you're dedicated to practicing safe sex despite this challenge is commendable. Just remember, even though it may take time and some trial and error to fi...

Quality can be defined as “a degree of excellence. In practice, we use testing as a measure of the outgoing quality of devices. The problem is that there is not a oneto-one correlation between test and quality. Just because a unit is tested doesn’t guarantee that it is defect-free. Similarly, “guaranteed-by-design” functionality, usually isn’t (with no offense intended to the incredibly talente...

The discovery of intelligence elsewhere would be of even greater import, as we have always considered humans to be special, even the pinnacle of Creation. To learn that there are others out there – others whose cognitive abilities exceed our own – would be a discovery that would recalibrate how our species views itself. It would also have consequences that can be only vaguely gauged, in much th...

There is a well-known argument from Leibniz’s Law for the view that coincident material things may be distinct. For given that they differ in their properties, then how can they be the same? However, many philosophers have suggested that this apparent difference in properties is the product of a linguistic illusion; there is just one thing out there, but different sorts or guises under which it...