Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for portable assays is driving the need for rapid, simple, low cost methods to prepare samples for downstream processing or detection. An important component of the sample preparation process is cell lysis. In this work, a simple microfluidic thermal lysis device is used to quickly release intracellular nucleic ...

The changes in bacteriolytic and proteolytic activities of skin cathepsins L and B were studied Ž . in thermal-stressed European eel Anguilla anguilla . When fish were kept in 108C water, all activities increased to reach maximum levels at Day 8 with varying increasing rates of 3.0 Ž . Ž . Ž . proteolysis , 4.2 lysis of FlaÕobacterium columnare , 5.7 lysis of Vibrio anguillarum and 7.8 Ž . lysi...

We have investigated whether nitrous oxide antagonizes or augments the CNS stimulant action of laudanosine in mice by comparing the mean convulsive doses (CD50 (SE] of a control group and those following pretreatment with 65% nitrous oxide in oxygen for 20 and 180 min. Nitrous oxide significantly increased CD50 from 46.8 (1.4) mg kg-1 of control to 57.3 (1.3) mg kg-1 at 20 min and 53.5 (1.7) mg...

An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targe...

Data on thermophysical properties are essential for modeling and evaluation of food processing operations involving heat transfer when safety, quality and energy cost are considered. Thermophysical properties of various meat and poultry emulsions were evaluated at 4 temperatures (20, 40, 60 and 80C). Thermal conductivities (0.26 W/mK to 0.48 W/mK) increased linearly with the temperature from 20...

A mutant cell strain derived from a Chinese hamster line by mutagenesis with ethylmethane sulfonate requires glycine for growth. In the wild type, glycine synthesis is catalyzed by serine hydroxymethyltransferase (EC 2.1.2.1). Cell fractionation by differential centrifugation and isopycnic sucrose gradient analysis reveals that the enzyme activity is found in both the mitochondrial and cytosol ...

Red Amaranth (Amaranthus tricolor) and green Amaranth (Amaranthus viridis) are two very important leafy vegetables consumed in most parts of India, not only for their flavours, but also for their pharmacological activities. The cooking processes are different in the different part of this country, and therefore, the present study was designed to analyze their in vitro antioxidant profile before...

Reteplase (rPA) is a thrombolytic agent used for the treatment of acute myocardial infarction. We studied the expression of rPA and its selected asparagine mutants after integration into the Pichia genome. Though methanol induction of the native and the rPA mutants showed similar expression levels (~200-250 mg/L), the mutants displayed significant loss of protease activity. Strikingly, the clot...

Time-resolved imaging was used to examine the use of pulsed laser microbeam irradiation to produce cell lysis. Lysis was accomplished through the delivery of 6 ns, lambda=532 nm laser pulses via a 40x, 0.8 NA objective to a location 10 microm above confluent monolayers of PtK2 cells. The process dynamics were examined at cell surface densities of 600 and 1000 cells/mm2 and pulse energies corres...

At low temperature, the synthesis of the colicin A lysis protein in Escherichia coli was slowed down, and consequently its functioning was retarded. The rates were restored when the bacteria were shifted for 10 min to 42 degrees C, except in an rpoH mutant, suggesting that one or more proteins regulated by sigma 32 is necessary for expression of colicin A lysis protein.