Adrenal steroid secretion by cats has been studied by Bush (1951, 1953) and reviewed by Dorfman (1959). The cat adrenal secretes both cortisol and corticosterone. Arteta & Borrell (1958) and Borrell (1958) have reported studies of urinary excretion of 17-hydroxy corticosteroids (PorterSilber chromogens). Cats appear to excrete in urine very low concentrations of 17-hydroxy corticosteroids, all ...

A spectrophotometric method for determining succinate dehydrogenase (SDH) activity assay in azide-sensitive yeast Saccharomyces cerevisiae has been developed. The permeabilization of yeast cells by 0.05 % digitonin permitted to study yeast enzymatic activity in situ. The reduction of blue tetrazolium salt (BT) to blue tetrazolium formazan (BTf) was conducted in the presence of phenazine methosu...

BACKGROUND AND PURPOSE Seizures cause cerebrovascular responses similar to those seen in conditions such as acute hypertension, ischemia/reperfusion, or fluid-percussion brain injury, which are associated with the generation of superoxide. Accordingly, we studied production of superoxide in experimental seizures. METHODS Superoxide production was measured in anesthetized cats equipped with do...

The potential antioxidant activity of diphenhydramine (histamine H1-receptor antagonist) and famotidine (histamine H2 receptor antagonist) was studied. Diphenhydramine inhibited the spontaneous, Fe(II)-induced and Fe(II)/ascorbate-induced lipid peroxidation, while famotidine showed a biphasic concentration-dependent effect on spontaneous lipid peroxidation (a stimulation by 1 mM and an inhibiti...

THE DETERMINATION of serum lactate dehydrogenase isoenzymes holds out numerous possibilities in the clinical laboratory aside from the obvious applications in myocardial immfarction and liver disease. An excellent review on different patterims obtained in various diseases can be found in an article by Wr#{243}blewski (1). Richterich et at. (2) describe the patterns found in humami tissues. Neve...

Tetrazolium salts can be used for measuring the reducing activity of microbial cultures colorimetrically, because the reduced form of these redox indicators is conspicuously colored (B. Jambor, Tetrazolium Salze in der Biologie, G. Fischer, Jena, 1960; B. J. Liska et al., J. Dairy Sci. 41:776, 1958). In an actively growing culture, the reducing activity is proportional with the number of bacter...