Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from acti...

Sulfur-containing nitriles have important research value in the life sciences due to their diverse biological activities resulting from the sulfur and cyano functional groups. Herein, a copper-catalyzed cyanothiolation of N-tosylhydrazones with thiocyanates to generate a-arylthioalkanenitriles bearing sulfur-substituted quaternary carbon center atoms has been described. This novel protocol invo...

The toxicity, physiologic effects, and antidotes are the points emphasized in the existing literature on the cyanides; Giacosa and Baumann alone have been interested in their fate in the body. Reports are unanimous that the aliphatic cyanides split off hydrocyanic acid, and that toxicity is directly proportional to the rate at which this splitting occurs, while an increase in the excretion of t...

This study of objective measurement of smoking behaviour reports the findings from a sample of 421 children aged 10 and 11 years from Sheffield. Saliva thiocyanate determination did not provide a satisfactory objective method of validating the self-reported smoking of children in this age group. There was a trend (non-significant) for non-smoking children from homes in which a close relative sm...