aquatic animals are important resources for extraction of valuable compounds such as enzymes. in this study, lipase was extracted from the intestine of rainbow trout. samples were defatted with acetone several times; ammonium sulphate (60%) was used for precipitation followed by ultrafiltration for concentrating the samples. the final purification of the enzyme was performed by passing of the s...

the present study was undertaken to screen the soil samples collected in iran for the presence of the bacillus subtilis lipase a gene. the bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (pcr). only four colonies were identified as putative b. subtilis strains and all contained the lipase a ge...

the objective of the present study was to determine the antilipidperoxidation and in vitro α-amylase and lipase inhibitory activity of the boswellia ovalifoliolata bal henry (bob) extract. the polyphenolic compounds, flavonoids, and tannins content of the extracts were estimated by spectrophotometry. antioxidant activity on goat liver lipid peroxidation and linoleic acid was determined.moreover...

conclusions there was a significant correlation between elevated lipase levels and the incidence of multiple organ failure. moreover, no significant association was found between the elevated amylase levels and organ failure. however, on admission, measurement of these enzymes coupled with routine laboratory investigations can be a powerful tool in the early detection of patients progressing to...

lipases catalyze hydrolysis and synthesis of triacylglycerols. these enzymes,especially the microbial ones, have wide commercial and industrial usage. bacillus subtilis lipase has interesting properties such as small size and tolerance to basic ph; therefore, developing techniques for its recognition and isolation is the focus of research in many laboratories. in the present study, two factors ...

Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of Isfahan province (Iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. To select a the...

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...