Tomato spotted wilt virus (TSWV) is transmitted in a persistent propagative manner by Frankliniella occidentalis, the western flower thrips. While it is well established that vector competence depends on TSWV acquisition by young larvae and virus replication within the insect, the biological factors associated with frequency of transmission have not been well characterized. We hypothesized that...

Tomato spotted wilt virus (TSWV) causes severe losses of tomato crops worldwide. To cope dynamically with such a threat, plants deploy strategies acting at the molecular and epigenetic levels. We found that symptoms progress in specific-genotype-manner upon TSWV infection. Susceptible genotypes showed within Auxin Response Factor (ARF8) promoter coupled to enhanced expression miRNA167a, reduced...

92 Journal of Plant Registrations, Vol 2, No. 2, May 2008 ‘Tifguard’ (Reg. No. CV-101, PI 651853) is a runnertype peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) cultivar that was released by the USDA-ARS and the Georgia Agricultural Experiment Stations in 2007. Tifguard was developed at the University of Georgia Coastal Plain Experiment Station, Tifton, GA, and was tested under the ...

Tomato spotted wilt virus (TSWV) has one negative sense (L) and two ambisense (M and S) RNAs. The lack of a tractable TSWV reverse genetics system has impeded direct demonstration of gene function and prevented the usual types of pathogen characterization. The TSWV NSm (non-structural) protein has been presumed to be the viral movement protein (MP) based on a few lines of indirect evidence. We ...

Twenty tomato spotted wilt virus (TSWV) isolates were serologically compared in ELISA employing five different procedures using a rabbit polyclonal antiserum against nucleocapsid proteins (NuAbR) and mouse monoclonal antibodies (MAbs), two directed to nucleocapsid proteins (N1 and N2) and four directed to glycoproteins G1 to G4. All the antisera were raised against TSWV-CNPH1. The 20 isolates w...