Glutathione peroxidase was extracted from calf trabecular meshwork. The kinetics of this enzyme were examined, varying the substrates hydrogen peroxide (H2O2), tert- butylhydroperoxide ( tBHP ), and glutathione. The activity of the enzyme in nonpurified homogenates was 596 nmole H2O2 reduced/min/gm wet weight and 680 nmole tBHP reduced/min/gm wet weight (27-28 nmole peroxide reduced/min/mg prot...

Core Concepts • iOP elevation results from increased outflow resistance in the outflow pathways: the trabecular meshwork (TM) and the uveoscleral pathway. • steady-state iOP is found in normal eyes where iOP builds up in response to inflow of aqueous humour to the level sufficient to drive fluid across the resistance of the outflow pathways. • in most glaucomatous eyes, the resistance of the ou...

PURPOSE To determine whether trabecular tissue in vivo and cultured trabecular cells have the messenger RNA transcript for transforming growth factor-beta 1 (TGF-beta 1), and to examine whether these cells synthesize and secrete TGF-beta 1 in vitro. METHODS Total RNA was isolated from the trabecular meshwork, iris, and ciliary body freshly excised from porcine eyes as well as from cultured tr...

PURPOSE To study the effects of JWH015, a CB2-selective agonist, on aqueous humor outflow facility, to investigate whether functional CB2 cannabinoid receptors are expressed in trabecular meshwork cells, and to study whether these receptors are involved in the enhancement of outflow facility induced by JWH015. METHODS A porcine anterior segment perfused organ culture model was used to measure...

A Biophysic Médical Nanolas Q-Switched pulsed neodymium-YAG laser has been used to produce lesions in human trabecular meshwork in vitro. Sectors of corneoscleral tissue containing trabecular meshwork were suspended in a waterbath which had had a Trokel gonioscopic contact lens mounted into one side by means of a watertight seal. The laser was used to produce lesions in the trabecular meshwork ...

BACKGROUND Glaucoma is one of the most common causes of blindness and is usually associated with elevated intraocular pressure. In patients with primary open angle glaucoma the number of trabecular meshwork cells is decreased. Death of the trabecular meshwork cells may be a result of apoptosis. OBJECTIVE To investigate the aqueous humor levels of soluble Fas (sFas) and Fas-Ligand (sFasL) in g...

PURPOSE To examine the adhesion of human trabecular meshwork cells with various extracellular matrix (ECM) proteins and to evaluate the roles and distribution of integrin receptors. METHODS Cultured human trabecular meshwork cells were added to 96-well plates either uncoated or coated with proteins including fibronectin, laminin, and vitronectin, as well as collagen types I, III, IV, V, and V...

PURPOSE Topical use of dexamethasone has long been associated with steroid induced-glaucoma, although the mechanism is unknown. We applied a strict filtering of comparative microarray data to more than 18,000 genes to evaluate global gene expression of cultured human trabecular meshwork cells in response to treatment with dexamethasone. METHODS Three human trabecular meshwork cell primary cul...