Using reverse transcription-PCR and molecular cloning, the complementary DNA sequence encoding preproinsulin-like growth factor I (IGF-I) of a teleost, the tilapia (Oreochromis mossambicus) was established from liver. At the amino acid level, tilapia IGF-I shows all residues necessary for the maintenance of tertiary structure and shares about 80% identity with IGF-I from other teleosts. The B a...

The effects of [Na(+)] or [K(+)] on Na, K-ATPase activity of FW-acclimated and SW-acclimated tilapia, puffer and milkfish were examined in gill homogenates. [Na(+)] or [K(+)] stimulated Na, K-ATPase hydrolyzing ATP in all experimental groups. ATP hydrolysis stimulated by [Na(+)] or [K(+)] followed Michaelian-Menten kinetics. Km values for [K(+)] (i.e., Km(K)), were lower in SW- than FW-acclimat...

To develop attenuated bacteria as potential live vaccines, sparfloxacin was used in this study to modify 40 isolates of Streptococcus agalactiae. Majority of S. agalactiae used in this study were able to develop at least 80-fold resistance to sparfloxacin. When the virulence of the sparfloxacin-resistant S. agalactiae isolates were tested in 10-12g Nile tilapia by intraperitoneal injection at d...

A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single myo-d-inositol 3-phosphate synthase (MIPS) transcript identified in all eel tissues, although two MIPS spliced variants [termed MIPS(s) and MIPS(l)] are found in all tilapia tissues. The larger tilapia transcript [MIPS(l)] results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic s...

Two experiments were conducted in 200-m earthen ponds at Asian Institute of Technology, Thailand, for 87 days to test the feasibility of an integrated pen-cum-pond system, which utilizes wastes from intensive culture of hybrid catfish (Clarias macrocephalus C. gariepinus) as nutrients for semi-intensive culture of Nile tilapia (Oreochromis niloticus). This integrated pen-cum-pond system enhance...

The extraction of mechanically separated meat has emerged as an attractive process. However, it increases the incorporation of oxygen and, consequently, of flavors due to rancidity. Thus, preservatives must be added. The objective of this study was to evaluate the shelf life of minced tilapia to replace synthetic preservatives with Hijiki and Nori seaweeds extracts. The application of the extra...

If you want to meet a truly tough fish, buy a ticket to Lake Magadi, Kenya. Here, the Magadi tilapia swim happily in the hot, alkaline waters of the lake, where oxygen levels soar and plummet daily and water chemistry pushes the boundaries of acid– base regulation. Few fish can survive such an extreme aquatic environment and this has attracted comparative physiologists for decades to the town o...

background: the current study tried to investigate the antioxidant and anti-bacterial effects of green tea and grape seed extract (with a volume of 2%) on durability of tilapia packed in polyethylene bags, which were kept in cool temperature of 4±1 °c. methods: prepared fish were divided into 3 batches: 2 batches were treated by dipping for 30 min in ethanolic of green tea extract (2% v/v) and ...

If you want to meet a truly tough fish, buy a ticket to Lake Magadi, Kenya. Here, the Magadi tilapia swim happily in the hot, alkaline waters of the lake, where oxygen levels soar and plummet daily and water chemistry pushes the boundaries of acid– base regulation. Few fish can survive such an extreme aquatic environment and this has attracted comparative physiologists for decades to the town o...

The acute toxicity of Roundup, a glyphosate herbicide, to Nile tilapia, Oreochromis niloticus, was investigated with emphasis on histopathological effects. The values of 24-h, 48-h, 72-h and 96-h LC50 for young tilapia were 17.5, 17.1, 16.9 and 16.8 ppm, respectively, and those for adult tilapia were 46.9, 44.4, 40.0 and 36.8 ppm, respectively. They indicated that adult fish were more tolerant ...