Escherichia coli UvrD protein is a 3' to 5' SF1 DNA helicase involved in methyl-directed mismatch repair and nucleotide excision repair of DNA. Using stopped-flow methods we have examined the kinetic mechanism of translocation of UvrD monomers along single-stranded DNA (ssDNA) in vitro by monitoring the transient kinetics of arrival of protein at the 5'-end of the ssDNA. Arrival at the 5'-end w...

uniparental disomy (upd) is a situation in which both members of a chromosome pair are inherited from one parent. this study has been conducted on a family with a five year-old healthy girl and a mentally retarded boy. the parents were first cousins and they both had robertsonian translocation between their long arm of chromosome 13 and 14 [45, xy t (13q14q)]. their affected son had a similar k...

a large number of simple or complex translocation involving the cml and aml chromosomal abnormalities has been described. this study was aimed to investigate the complex chromosome aberrations in the series of myeloid malignancies including cml and aml. the present report deals analyzed 187 consecutive with cml and aml patients, using methotrexate  cell synchronization and  un-stimulated cultur...

background: carriers of translocations may have an increased risk of an unbalanced progeny due to imbalances and delays in meiosis. case: a 24-year-old pregnant iranian female was referred to the genetic department of yazd clinical and research centre for infertility because of her pregnancy history. she had three previous pregnancies, two of which ended in abortion. the one live born infant wa...

Type I restriction enzymes bind to a specific DNA sequence and subsequently translocate DNA past the complex to reach a non-specific cleavage site. We have examined several potential blocks to DNA translocation, such as positive supercoiling or a Holliday junction, for their ability to trigger DNA cleavage by type I restriction enzymes. Introduction of positive supercoiling into plasmid DNA did...

heavy metals such as cadmium mainly enter in the environment and ecosystem as a result of human activities. this study was conducted in order to evaluate the ability of bacterial inoculants to enhance efficiency of phytoextraction in a calcareous soil. three plants (amaranthus retroflexus, helianthus annus and medicago sativa), along with three levels of inoculants and four levels of cd were ev...

plant-based remediation (i.e. phytoremediation) is one of the most significant eco-sustainable techniques to cope with devastating consequences of pollutants. in the present study, the potential of a wetland macrophyt (i.e. potamogeton crispus ) for the phytoremediation of heavy metals (i.e. cu, cr, pb, as and cd) in the anzali wetland was evaluated. the results showed that p. crispus tends to ...

in the present study, a field study was performed on some herbaceous plants growing in thesoutheast of ahvaz, where some metal producing industries are active. the aim of this studywas to investigate and compare manganese (mn) and zinc (zn) accumulation in seven dominantherbaceous plants in this area. plant samples were collected randomly. associated soils weresampled from the same sites next t...