Phenoloxidase (Po) activity was measusred spectrophotometrically in serum, plasma and haemocytes (HLS) of the freshwater crayfish (Pontastacus leptodactylus). The highest activity was found in HLS suggesting that the haemocytes are the major source of the Po or its proform prophenoloxidase (proPO) in crayfish. Furthermore, the enzyme activity in serum samples was reduced after freezing the samp...

Phenoloxidase (Po) activity was measusred spectrophotometrically in serum, plasma and haemocytes (HLS) of the freshwater crayfish (Pontastacus leptodactylus). The highest activity was found in HLS suggesting that the haemocytes are the major source of the Po or its proform prophenoloxidase (proPO) in crayfish. Furthermore, the enzyme activity in serum samples was reduced after freezing the samp...

For expressing trypsin inhibitor activity (TIA), units inhibited (TUI), inhibited, and inhibitors have been used. Although the last two are preferred, their calculations in current practices require refinement. With proposed AOCS method Ba 12a-2020, four experiments were conducted, using preparations having specific of 11,625, 12,602, 13,728, 14,926 N?-benzoyl-L-arginine ethyl ester (BAEE) unit...

Protein digestion by trypsin has been widely used in many industrial and research applications. However, extensive use of trypsin is limited because of the rapid decrease in enzymatic activity caused by autolysis at optimal pH and temperature. To improve the enzymatic performance of trypsin, we synthesized highly stabilized polymer–trypsin conjugates using vinylmethylether-maleic acid copolymer...

The changes in serum trypsin concentration have been measured in 47 subjects for up to 2 hours after a Lundh meal. In 18 healthy controls, mean fasting trypsin concentration was 285 +/- 125 ng/ml (mean +/- 2 SD). The maximum increase after the Lundh meal (the trypsin response ratio) was 6.7 +/- 7.5%. Six patients with chronic renal failure had elevated fasting serum trypsin concentrations (rang...