background: adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines.  it has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer.  this study has managed to investigate endogenous and/or exogenous expression of visfatin and resisti...

background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...

many cell lines, when cultured under proper conditions, can form three dimensionalstructures called multicellular spheroids. these spheroids resemble in vivo tumor models in severalaspects. therefore, studying growth characteristics and behavior of spheroids is beneficial inunderstanding the behavior of tumors under various experimental conditions. in this work, we havestudied the growth proper...

The origin of ovarian epithelial tumors is still indistinct. Müllerian and coelomic hypothesis are controversial. Recently, there is also a small number of reports that tubo-peritoneal junction may play an important role in the development of tubo-ovarian tumors. Therefore, we aimed to investigate the relationship between tumors originating from Mullerian epithelium and peritoneal mesothelium, ...

OBJECTIVE The chemotherapy response score (CRS) system based on histopathological examination has been recently proposed for tubo-ovarian high-grade serous carcinoma (HGSC) to assess response to neoadjuvant chemotherapy (NAC). This study was aimed at validating the CRS system in an external cohort of tubo-ovarian HGSC patients. METHODS This study included 110 tubo-ovarian HGSC patients who un...

the lung cancer is one of the most dangerous cancers and is also the leading cause of cancer death worldwide, accounting for about 1.3 million deaths annually. however in clinical practice, lung cancer therapies commonly do with chemotherapy, although it is hard because the lung cancer may progress to metastasis stage. the metastasis of lung cancer is highly dependent of expression of matrix me...

background- cd24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. our aim was clarify correlation between cd24 expression and camp:cgmp ratio in murine colorectal cancer cell line (ct26) after using cholera toxin. materials and methods- the ct26 cells were cultured in microtubes for assaying camp and cgmp; also the cells were cultured in flasks for ass...

the sporozoites of theileria annulata invade bovine mhc ii cells, where they differentiate into schizonts. the later can immortalize and induce fundamental changes in their host cells. live attenuated vaccine is an important way of controlling t. annulata infection of cattle. production is by prolonged cultivation of macroschizont-infected cells. the mechanisms underlying this transformation ar...

methods s. cerevisiae was cultured and then disrupted by sonication. after centrifugation, the harvested supernatant was considered to be a cytoplasmic extract. the protein concentration was determined by the biuret method and the extract was diluted to concentrations of 500, 1000, and 2000 µg protein/ml. the frequencies of apoptosis and necrosis were assessed in extract-treated k562 cells by e...

background: the adjuvanticity potential of lactobacillus casei was first suggested in an old survey. the present study was designed to investigate the efficacy of a new immunotherapy against breast cancer made by mixing an extract of heated 4t1 mammary carcinoma cell line and a heat-killed preparation of lactobacillus casei. methods: female balb/c mice (6–8 weeks old, n=40) were challenged subc...