Pyrrolidone carboxyl peptidase (Pcp) is an aminopeptidase (EC 3.4.11.8) able to specifically remove the L-pyroglutamyl residue from the amino-terminus of polypeptides. Since nothing was known concerning the regulation and function of Pcps, a mutant of a milk-isolated strain lacking Pcp activity (Pseudomonas fluorescens MB1), was constructed by homologous recombination using a transcriptional fu...

In leaves of most C(4) plants, the biochemistry of photosynthesis is partitioned between mesophyll and bundle sheath cells. In addition, their cell biology and development also differs from that in C(3) plants. We have a poor understanding of the mechanisms that generate the cell-specific accumulation of proteins used in the C(4) pathway, and there are few genes that have been shown to be impor...

beta-Glucuronidase (uidA) reporter gene fusions were constructed for the hrpZ, hrpL, and hrpS genes from the phytopathogen Pseudomonas syringae pv. maculicola strain ES4326. These reporters, as well as an avrRpt2-uidA fusion, were used to measure transcriptional activity in ES4326 and a ES4326 rpoN mutant. rpoN was required for the expression of avrRpt2, hrpZ, and hrpL in vitro in minimal media...

Antibiotic-resistance genes carried by coliforms in drinking water is a concerning issue for public health Bangladesh. This research was out to identify and understand the importance of int1 gene spread resistance bacterial antibiotics through consumption contaminated water. A total 31 samples were collected from restaurants ( n = 18), center 9), residences 4) located Chattogram City, The isola...

A transgenic winter triticale line expressing the uidA gene, encoding ?-glucuronidase, was used to assess pollen flow in field experiments over two consecutive vegetation seasons central Poland. The experimental design included variants of mixed and non-transgenic lines. Pollen grains were collected using passive traps located at 0, 10, 30, 60 85 m from line. GM detected histochemically by stai...

Root system architecture is a major determinant of water and nutrient acquisition as well as stress tolerance in plants. The Mediator complex is a conserved multiprotein complex that acts as a universal adaptor between transcription factors and the RNA polymerase II. In this article, we characterize possible roles of the MEDIATOR8 (MED8) and MED25 subunits of the plant Mediator complex in the r...

Root system architecture is a major determinant of water and nutrient acquisition as well as stress tolerance in plants. The Mediator complex is a conserved multiprotein complex that acts as a universal adaptor between transcription factors and the RNA polymerase II. In this article, we characterize possible roles of the MEDIATOR8 (MED8) andMED25 subunits of the plant Mediator complex in the re...

Plant specific cell cycle regulation in response to developmental and environmental signals was investigated at the level of gene expression in the model plant Arabidopsis thaliana (L.) Heynh. Specific attention was paid to the regulation of the mitotic cyclin CYCB1;1, by means of a mutant approach, during lateral root development and in salt stress conditions. Analysis of the activity of a GUS...

Multiplex PCR amplification of lacZ, uidA and plc genes was developed for the simultaneous detection of total coliform bacteria for Escherichia coli and Clostridium perfringens, in drinking water. Detection by agarose gel electrophoresis yielded a band of 876 bp for the lacZ gene of all coliform bacteria; a band of 147 bp for the uidA gene and a band of 876 bp for the lacZ gene of all strains o...