نتایج جستجو برای: universal rice primer
تعداد نتایج: 207599 فیلتر نتایج به سال:
Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for theBacteria domain able to amplify the anammox 16S rRNA genes. Ametagenomic analysis (pyrosequencing) of total bacterial diversity including anammox population in f...
Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for the Bacteria domain able to amplify the anammox 16S rRNA genes. A metagenomic analysis (pyrosequencing) of total bacterial diversity including anammox population in...
Three tuberculous, twenty-one non-tuberculous mycobacterial (NTM) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of DNA fragments in a polymerase chain reaction with single universal primer (UP-PCR). A rather wide limit of tolerance for variations in procedure of PCR mixture preparation and thermocycling parameters was found. There...
Salt stress is one of the main abiotic stresses for rice that causes negative effects on its growth and productivity. In present study, effects of salt stress on differential gene expression of some genes which are responsible in salt stress were investigated in two rice tolerant and sensitive genotypes (FL478 and IR29) by applying cDNA-AFLP technique. Among the TDFs (Transcript Derived F...
Real-time quantitative polymerase chain reaction (qPCR) is one of the most important methods for analyzing the expression patterns of target genes. However, successful qPCR experiments rely heavily on the use of high-quality primers. Various qPCR primer databases have been developed to address this issue, but these databases target only a few important organisms. Here, we developed the qPrimerD...
We developed a multiplex polymerase chain reaction (PCR) coupled with ligation detection reaction and universal array system for screening genetically modified rice and further identifying specific transgenes in rice. Three common elements in transgenic rice (CaMV 35S promoter, FMV 35S promoter and nos terminator) with species and internal controls (rice actin, 18S rRNA) were used for screening...
BACKGROUND We have developed a novel, microsphere-based universal array platform referred to as the Tag-It platform. This platform is suitable for high-throughput clinical genotyping applications and was used for multiplex analysis of a panel of thrombophilia-associated single-nucleotide polymorphisms (SNPs). METHODS Genomic DNA from 132 patients was amplified by multiplex PCR using 6 primer ...
Bipolaris oryzae causes rice brown spot disease which reduces rice yield with substantial global impact. Streptomycetes isolated from rice fields of Guilan Province, Iran, showed antifungal activity against the tested pathogen. From five active Streptomyces spp. isolates, isolate G showed high antagonistic activity. To determine its taxonomical identity, its colonies character...
Bipolaris oryzae causes rice brown spot disease which reduces rice yield with substantial global impact. Streptomycetes isolated from rice fields of Guilan Province, Iran, showed antifungal activity against the tested pathogen. From five active Streptomyces spp. isolates, isolate G showed high antagonistic activity. To determine its taxonomical identity, its colonies character...
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.
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