background : evaluation of exposition to volatile organic compounds (voc) and compare urinary level of urinary biological exposure index in sweepers in west of iran . methods: this study was carried out on 40 street sweepers and 40 non-exposed as control group living in hamadan city, west of iran. voc was analyzed using gas chromatography equipped with a flame ionization detector (fid). the uri...

The relationship between 114 cases with decreased enzymatic activities of mitochondrial respiratory chain (MRC) complexes I-V (C I-V) in muscle and metabolites in urine and plasma was retrospectively examined. Less than 35% disclosed abnormal plasma amino acids and acylcarnitines, with elevated alanine and low free carnitine or elevated C4-OH-carnitine as the most common findings, respectively....

We have determined that the organic matrix of calcium oxalate kidney stones contains a glycoprotein inhibitor of calcium oxalate crystal growth (nephrocalcin) that resembles nephrocalcin present in the urine of patients with calcium oxalate stones and differs from nephrocalcin from the urine of normal people. Pulverized calcium oxalate renal stones were extracted with 0.05M EDTA, pH 8.0; nephro...

er than the normal amount. Especially, it is interesting that nicotinic acid reduces serum cholesterol and triglycerides.i) To study the unknown mechanism, the establishment of a practical method to measure nicotinic acid and its direct metabolite, nicotinuric acid, is important. In l978, Hengen et al.2] measured nicotinic acid and nicotinuric acid in plasma and urine with reversed-phase high-p...

BACKGROUND Volatile organic compounds (VOCs) can be intermediates of metabolic pathways and their levels in biological samples may provide a better understanding about diseases in addition to potential methods for diagnosis. Headspace analysis of VOCs in urine samples using solid phase micro extraction (SPME) coupled to gas chromatography - mass spectrometry (GC-MS) is one of the most used tech...

Acidosis is an important cause of mortality in severe falciparum malaria. Lactic acid is a major contributor to metabolic acidosis, but accounts for only one-quarter of the strong anion gap. Other unidentified organic acids have an independent strong prognostic significance for a fatal outcome. In this study, a simultaneous bio-analytical method for qualitative and quantitative assessment in pl...

Radioimmunoassay of urinary or plasma aldosterone requires an initial extraction of steroids before purification and analysis. This extraction characteristically must be performed on acid-hydrolyzed urine or plasma, both of which have been supplemented with tnitiated aldosterone, to assess the analytical recovery. After extraction, the organic phase must be washed sequentially with 0.1 mol/lite...

In the literature on the estimation of urinary lead, it is usually recommended that not less than 100 ml. aliquots of the urine sample be taken for analysis. This has certain disadvantages, not the least of which is the time required to ash the organic content of the urine. In addition, spot samples of urine collected from groups of workmen are often less than the 200 ml. volume required for du...

Methotrexate-3H (MTX-3H) was accumulated against a con centration gradient by renal cortical slices. This accumulation was energy dependent, inhibited by metabolic inhibitors, Diamox, and high concentrations of ouabain. Several folie acid analogs containing the pteridine ring were inhibitors of this accumulation while certain weak organic acids were less striking inhibitors. This suggests that ...