conclusions mtb32c and hbha genes were successfully isolated from h37rv genome and cloned into an eukaryotic expression vector. this vector can be considered as a vaccine to evaluate immune responses in animal models. results in the current study, recombinant pcdna3.1 (+) vector containing mtb32c and hbha genes was successfully constructed. the desired size of dna fragment was observed using si...

background whooping cough is caused by bordetella pertussis, and it remains a public health concern. whole-cell pertussis vaccines have been commonly employed for expanded immunization. there is no doubt of the efficacy of whole cell pertussis vaccine, but it is necessary to improve the vaccine to decrease its toxicity. objectives in this study, an inactivation process of dealing with pertussis...

Background: Potency evaluation of rabies vaccine is a cheap, fast, high precision and consistent with ethical values is critical, so researchers have modified a variety of methods such as: National Institute of Health (NIH) method, Single Radial Immunodiffusion (SRID) and so on. The purpose of the present study was to replace an in vitro method consistent with medical ethics criteria instead of...

dna vaccination has been developed as a noteworthy immunotherapeutic approach for the battle against many serious challenges to human and animal safety, including infectious illnesses, autoimmunity, allergy and cancer. clinical trials prepare chances to examine whether dna vaccines can fulfill their final aim of demonstrating efficiency in treating humans. today, anticancer dna vaccines are qui...

Avian Pasteurella multocida is an agent of fowl cholera. The protective effect achieved through orthodoxvaccines is not ideal. The research on novel vaccines against avian Pasteurella multocida is imperative. Inthis study, the genes encoding outer membrane protein H and A (OmpH and OmpA) were cloned into theeukaryotic expression vector pcDNA3.1(+) and the recombinant plasmids, namely DNA vaccin...

avian pasteurella multocida is an agent of fowl cholera. the protective effect achieved through orthodoxvaccines is not ideal. the research on novel vaccines against avian pasteurella multocida is imperative. inthis study, the genes encoding outer membrane protein h and a (omph and ompa) were cloned into theeukaryotic expression vector pcdna3.1(+) and the recombinant plasmids, namely dna vaccin...