The G protein-coupled receptor (GPCR) dimer interface plays an important role in the formation and stabilization of dimer. Therefore, identifying potential receptor-receptor is part studying GPCRs. Various strategies have been employed to study GPCR explore its functional significance, but experimental methods lack robustness calculations are laborious. Herein, we report a combined optimized ca...

Vasopressin and its receptors modulate several gut functions, but their role in intestinal inflammation is unknown. Our aims were to determine 1) the localization of V1b receptors in human and rodent colon, 2) the role of vasopressin and V1b receptors in experimental colitis using two approaches: V1b⁻(/)⁻ mice and a selective V1b receptor antagonist, SSR149415, and 3) the mechanisms involved. V...

To determine whether receptor-mediated endocytosis occurs in vasopressin-responsive cells, we developed a model system using synthetic fluorescent-labeled vasopressin analogs and A10 (smooth muscle) and LLC-PK1 (kidney epithelial) cells in culture; these cell lines express V1 and V2 vasopressin cell surface receptor types, respectively. We used epifluorescence microscopy to examine the binding,...

Vasopressin is one of several small neuropeptides that are reported to be autocrine growth factors for small cell carcinoma of the lung (SCCL). It has been assumed that this peptide exercises its ntitogenic influences through the Vasopressin V,., receptor, and we have previously demon strated that this receptor is expressed by classical and variant SCCL. Activation of the vasopressin Vla recept...

Vasopressin-stimulated insertion of the aquaporin 2 (AQP2) water channel into the plasma membrane of kidney collecting duct principal cells is a key event in the urinary concentrating mechanism. The paradigm for vasopressin-receptor signaling involves cAMP-mediated protein kinase A activation, which results in the functionally critical phosphorylation of AQP2 on amino acid serine 256. We previo...

Sato, Yoshihiko, Hiroyuki Hanai, Atsuhiro Nogaki, Koki Hirasawa, Eizo Kaneko, Hisayoshi Hayashi, and Yuichi Suzuki. Role of the vasopressin V1 receptor in regulating the epithelial functions of the guinea pig distal colon. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G819–G828, 1999.—Vasopressin has a wide spectrum of biological action. In this study, the role of vasopressin in regula...

Abstract Background and Aims Poly(ADP-ribosy)lation (PARylation), which is mediated by poly(ADP-ribose) polymerases (PARPs), catalyzes the transfer of ADP-ribose from NAD+ molecules to acceptor proteins, regulates diverse cellular processes. Since PARP1 gene-deficient mice revealed an increase in urine volume, we aimed examine role PARP1, most abundant type protein PARPs family, vasopressin-med...

Specific binding sites for3H-arginineQasopressin (AVP) have been characterized in rat septal membranes. Scatchard analyses revealed a single class of high-affinity binding sites having an equilibrium dissociation constant of 1.7 f 0.3 nM and total binding capacity of 22.6 k 4.2 fmol/mg protein. Binding displacement studies with peptide analogs of AVP indicate that this binding site is similar t...